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Rat monoclonal [LO-MM-9] Anti-Mouse IgM heavy chain (Biotin) (ab11659)

Submit a review Q&A (1)

Key features and details

  • Rat monoclonal [LO-MM-9] Anti-Mouse IgM heavy chain (Biotin)
  • Conjugation: Biotin
  • Host species: Rat
  • Isotype: IgG2a
  • Suitable for: ELISA

製品の概要

  • 製品名

    Rat monoclonal [LO-MM-9] Anti-Mouse IgM heavy chain (Biotin)
    IgM 二次抗体 製品一覧

  • 由来種

    Rat

  • ターゲット生物種

    Mouse

  • 特異性

    ab11659 recognises the mu chain of mouse IgM.

  • アプリケーション

    適用あり: ELISAmore details

  • 免疫原

    Polyclonal and monoclonal mouse IgM.

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Purified IgM

  • 特記事項（精製）

    Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B.

  • ポリ/モノ

    モノクローナル

  • クローン名

    LO-MM-9

  • ミエローマ

    IR983F

  • アイソタイプ

    IgG2a

  • 特記事項

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11659の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use a concentration of 0.25 µg/ml.
特記事項
ELISA
Use a concentration of 0.25 µg/ml.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab11659 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11659 は論文での使用が確認できていません。

レビューと Q&A

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Question

I am working with B cells which express an human IgG or human IgM as well as a murine IgM. I would like to show the expression of the murine IgM in FACS. Therefore I need an antibody which ONLY recognizes the murine IgM but not the human IgM or IgG. In your catalogue I found an "Mouse IgM secondary antibody - µ chain speciic- F(ab)2 Fragment (ab5932). Do you know if this antibody crossreacts with human IgM/G? Unfortunately this antibody is coupled to PE. Since my cells are GFP positive I prefere Biotin or APC labeled antibodies. Do you have an anti mouse-µ antibody not reacting with human IgG/M which is coupled to Biotin or APC?

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Answer

We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cross adsorbed or tested against human IgM, and we cannot guarantee their suitable for your assay. Two other biotin conjugated anti-mouse IgG antibodies but only tested in ELISA: ab11659 Mouse IgM secondary antibody [LO-MM-9] - Heavy Chain specific. Unable to guarantee non-reactivity with human Ig. ab49760 will not recognise other mouse Ig's as there was no reaction against IgA, IgG, and IgM. Human IgG or IgM cross-reactivity has not been tested.

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