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Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain (ab11566)

Submit a review Q&A (1)References (1)

Key features and details

  • Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain
  • Host species: Rat
  • Isotype: IgG1
  • Suitable for: ELISA

製品の概要

  • 製品名

    Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain
    IgG2b 二次抗体 製品一覧

  • 由来種

    Rat

  • ターゲット生物種

    Mouse

  • 特異性

    ab11566 recognises murine IgG2b, binding to the gamma 2b heavy chain.

  • アプリケーション

    適用あり: ELISAmore details

  • 免疫原

    Full length protein corresponding to Mouse IgG2b. Polyclonal IgG from BALB/c mice.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified IgG prepared by affinity chromatography.

  • ポリ/モノ

    モノクローナル

  • クローン名

    LO-MG2b-1

  • ミエローマ

    IR983F

  • アイソタイプ

    IgG1

  • 特記事項

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11566の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
特記事項
追加情報
ELISA (coating): Use at a concentration of 5 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab11566 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11566 は 1 報の論文で使用されています。

  • Lau YF  et al. The magnitude of local immunity in the lungs of mice induced by live attenuated influenza vaccines is determined by local viral replication and induction of cytokines. J Virol 85:76-85 (2011). PubMed: 20962087

レビューと Q&A

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Question

Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. As proof of concept, I’d like to detect known isotype control samples using the pair of antibodies that I have. Now, I’m interested in ab18421 ([MG2b-57]) and ab18469 ([MPC-11]). Which one do you recommend? Are both isotype control samples recognized by the antibodies that I have? Another question I’d like to ask is what is the meaning of clone numbers like MG2b-1 and MG2b-2? Can I know the epitope information from these numbers? Thank you.

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Answer

Thank you for your enquiry.


Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

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