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Rat monoclonal [LO-MG1-2] Anti-Mouse IgG1 heavy chain (Biotin) (ab11587)

Submit a review Q&A (2)References (3)

Key features and details

  • Rat monoclonal [LO-MG1-2] Anti-Mouse IgG1 heavy chain (Biotin)
  • Conjugation: Biotin
  • Host species: Rat
  • Isotype: IgG1
  • Suitable for: ELISA

製品の概要

  • 製品名

    Rat monoclonal [LO-MG1-2] Anti-Mouse IgG1 heavy chain (Biotin)
    IgG1 二次抗体 製品一覧

  • 由来種

    Rat

  • ターゲット生物種

    Mouse

  • 特異性

    ab11587 binds to the heavy chain of murine IgG1.

  • アプリケーション

    適用あり: ELISAmore details

  • 免疫原

    Full length protein corresponding to Mouse IgG1. Purified mouse IgG1 from BALB/c mice

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified IgG prepared by affinity chromatography.

  • ポリ/モノ

    モノクローナル

  • クローン名

    LO-MG1-2

  • ミエローマ

    IR983F

  • アイソタイプ

    IgG1

  • 特記事項

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11587の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
特記事項
追加情報
ELISA: Use at a concentration of 0.125 µg/ml, as detecting antibody.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab11587 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11587 は 3 報の論文で使用されています。

  • Bergman ML  et al. Tolerogenic insulin peptide therapy precipitates type 1 diabetes. J Exp Med 214:2153-2156 (2017). PubMed: 28536239
  • Daniel C  et al. Prevention of type 1 diabetes in mice by tolerogenic vaccination with a strong agonist insulin mimetope. J Exp Med 208:1501-10 (2011). ELISA ; Mouse . PubMed: 21690251
  • Babaya N  et al. Murine high specificity/sensitivity competitive europium insulin autoantibody assay. Diabetes Technol Ther 11:227-33 (2009). ELISA . PubMed: 19344197

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question



Inquiry: This antibody is described as an anti-IgG1 mAb. Although IgG1 was the immunogen used and it would bind IgG1, from the information provided it is not clear whether this antibody has been tested for binding to other subclasses of IgG (e.g. IgG2a, IgG2b, IgG3) or to IgA, IgM, or IgE. Has reactivity to other Ig classes/subclasses been tested and is it specific only to IgG1? thanks Colin

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Answer

Thank you for contacting us.


Our rat anti mouse IgG1 heavy chain antibody, recognizesmouse IgG1, and does not bind to other mouse immunoglobulin classes (such as IgM) or subclasses (such as IgG2a).





I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

My ELISA is not working.

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Answer

Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Below please find the link to the questionnaire we discussed over the phone. Once you have submitted the form to us, we will look at the protocol you used and see if there are any suggestions we can make to improve your results. If we determine that the antibody is not working as stated on the datasheet, and you have purchased it in the last 90 days, we would be happy to replace or refund the antibody for you. The questionnaire can be found at: https://www.abcam.com/index.html?section=elisa&pageconfig=technical&intAbID=13781&mode=questionaire I look forward to receiving your reply.

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