Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
Key features and details
- Rabbit Anti-Sheep IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr
Related conjugates and formulations
製品の概要
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製品名
Rabbit Anti-Sheep IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Sheep -
アプリケーション
適用あり: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details -
免疫原
Sheep IgG whole molecule
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Horseradish Peroxidase (HRP).
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研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6747の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/1000 - 1/4000. PubMed: 15319485
|
| ELISA |
Use at an assay dependent dilution.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500 - 1/2500.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/4000. PubMed: 15319485 |
|
ELISA
Use at an assay dependent dilution. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/2500. |
|
IHC-Fr
Use at an assay dependent concentration. |
画像
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Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)Kälble F et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016).Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar: 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (55)
ab6747 は 55 報の論文で使用されています。
- Wang W et al. pH-Responsive Delivery of H2 through Ammonia Borane-Loaded Hollow Polydopamine for Intervertebral Disc Degeneration Therapy. Oxid Med Cell Longev 2023:7773609 (2023). PubMed: 36778204
- Wang K et al. Silencing circular RNA hsa_circABCC1 inhibits osteosarcoma progression through down-regulating HDAC4 via sponging miR-591. Environ Toxicol 38:1565-1576 (2023). PubMed: 37052456
- Simpson SJ et al. Efficacy of a synthetic peptide Chlamydia pecorum major outer membrane protein vaccine in a wild koala (Phascolarctos cinereus) population. Sci Rep 13:15087 (2023). PubMed: 37699951
- Li J et al. Overexpression of PFKFB3 promotes cell glycolysis and proliferation in renal cell carcinoma. BMC Cancer 22:83 (2022). PubMed: 35057732
- Chen L et al. Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury. J Nanobiotechnology 20:210 (2022). PubMed: 35524268
