Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I would like to clarifya few points.The primaryantibody used to detectUCP1 israised in rabbit. It is arabbit polyclonalantibody.In order to detect this efficiently, an anti-rabbit antibodywould need to be used.The antibody ab6734 is ananti-rat antibodywhich is unlikely tobe able to detect therabbit IgG of ab10983.I would suggest using an antibody such ashttps://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97051.html.Was the antibody ab6734 used with any Rat primary antibodies with difficulty?

Thank you for your cooperation and look forward to your reply.

I would like to reassure you that these products are safe to use.

The anti-Fibrillarin antibody [38F3] is a tissue culture supernatant and is not purified. Other proteins will be within the solution and this can give it an off white colour. The secondary antibody is conjugated to HRP which can also be coloured due to the HRP conjugate. This is likely to be unconjugated HRP that has not been removed. There will be batch to batch variation with both products and this may be why you have not observed this before.

We ship the majority of our products with an ice pack during shipment to maintain a temperature of ˜4C. However if this is not suitable for the product we will ship on either dry ice or at room temperature.

Please continue to use these products as advised and if you do experience any problems please do not hesitate to contact us. You will be covered by our Abpromise guarantee so if the products do not perform as described you will be eligible for a full refund/replacement.

Just let me know if you have further questions.

Thank you for your enquiry. I have received a reply from the originator of ab6734 and ab7104. For both products, there are approximately two HRPs per antibody. The method we use for our HRP conjugation is proprietary. ab6734 is only tested against rat IgG and cross reactivity is possible, especially with species that are similar such as mouse and other small animals. Product ab7104 is preadsorbed against human serum proteins and shows no cross reactivity with human serum proteins. However, this may cross react with similar species as well as this has not been tested. Lastly, the concentrations listed on the data sheet are the concentration of the HRP conjugated antibody. I hope this information helps. Please don't hesitate to contact us again if you have further questions.

Thank you for your email. Our source for this antibody tested ab11564 in Western blotting with recombinant TNF alpha. Conditions were standard. To use this antibody in Western blotting, I would recommend referring to our general Western blot protocol which can be found in the protocol section of the online datasheet. I would suggest using ab11564 at a 1:1000 dilution, and ab6734 at a 1:5000 dilution. I conferred with our source for ab11564 and it sounds like you are detecting the membrane bound form of TNF alpha (the 26 kDa integral membrane protein). If you have any additional questions, please contact us again.