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Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)

Submit a review Q&A (3)References (28)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)

Key features and details

  • Rabbit Anti-Rat IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase beta-galactosidase FITC HRP Unconjugated Unconjugated

製品の概要

  • 製品名

    Rabbit Anti-Rat IgG H&L (Biotin)
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Rat

  • アプリケーション

    適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • 免疫原

    Rat IgG whole molecule

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6733の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent dilution.
ELISA
1/500000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent dilution.
ELISA
1/500000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
    Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
    Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
    Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (28)

ab6733 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6733 は 28 報の論文で使用されています。

  • MaruYama T  et al. Pyrolyzed deketene curcumin controls regulatory T cell generation and gastric cancer metabolism cooperate with 2-deoxy-d-glucose. Front Immunol 14:1049713 (2023). PubMed: 36814928
  • van Elsas MJ  et al. Invasive margin tissue-resident macrophages of high CD163 expression impede responses to T cell-based immunotherapy. J Immunother Cancer 11:N/A (2023). PubMed: 36914207
  • Hutchison ER  et al. Dissecting the impact of dietary fiber type on atherosclerosis in mice colonized with different gut microbial communities. NPJ Biofilms Microbiomes 9:31 (2023). PubMed: 37270570
  • Kasahara K  et al. Gut bacterial metabolism contributes to host global purine homeostasis. Cell Host Microbe 31:1038-1053.e10 (2023). PubMed: 37279756
  • Nakagomi E  et al. Cancer stem cell markers CD44v9+/CD133- are associated with low apoptosis in both sporadic and ulcerative colitis-associated colorectal cancers. Histol Histopathol 37:587-595 (2022). PubMed: 35224715
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

background with ab6733 on Mouse tissue samples

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Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab6733 que vous avez reçu ne fonctionne pas comme attendu.
Comme convenu j'ai mis en place l'envoi d'une unité de ab7103 (https://www.abcam.com/ab7103), le numéro de commande de remplacement gratuit de ce produit est *****.
Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

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Question

Customer kindly requested Biotin conjugated secondary antibodies for ab2378, ab5690, ab103573, ab10558, ab955, ab53444, ab9018

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Answer

Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044.html ab5690, ab103573, ab10558 are rabbit polyclonal antibodies. I would recommend using ab6720; https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6720.html ab53444 is a rat monoclonal antibody. I would recommend using ab6733; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6733.html To let you know we are running a promotion '4 for 3' on primaries; if you could select 1 more primary antibody then you will be entitled to 8 primary antibodies for the price of 6. Use the discount code and follow the instructions given on website. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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Question

Is this or any other anti-rat Ab absorbed to prevent cross reactivity with mouse Abs ? I need to run a double label withj mouse and rat Abs and I need to prevent any cross reactivity. I can't use any rabbit Abs for another technical reason. Thanks David

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Answer

I recommend ab7096 -- Goat polyclonal to rat IgG H&L (biotin) Pre-Adsorbed

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