Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735)
Key features and details
- Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr
Related conjugates and formulations
製品の概要
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製品名
Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Rat -
アプリケーション
適用あり: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details -
免疫原
Rat IgG whole molecule
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標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
ポリ/モノ
ポリクローナル -
特記事項
Part of the AbExcel range. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6735の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/5000 - 1/50000.
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ELISA |
Use at an assay dependent dilution.
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Immunomicroscopy |
Use at an assay dependent concentration.
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|
Dot blot |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/200 - 1/1000.
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|
IHC-Fr |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/5000 - 1/50000. |
ELISA
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
IHC-Fr
Use at an assay dependent concentration. |
画像
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All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/2000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 5-6 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab6735 は論文での使用が確認できていません。