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Rabbit Anti-Pig IgG H&L (Texas Red ®) (ab6775)

Submit a review Q&A (1)References (5)

Key features and details

  • Rabbit Anti-Pig IgG H&L (Texas Red ®)
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Rabbit
  • Suitable for: WB, Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

製品の概要

  • 製品名

    Rabbit Anti-Pig IgG H&L (Texas Red ®)
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Pig

  • アプリケーション

    適用あり: WB, Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

  • 免疫原

    pig IgG whole molecule

  • 標識

    Texas Red ®. Ex: 596nm, Em: 620nm

製品の特性

  • 製品の状態

    Lyophilized:Reconstitute with 1 ml of water.

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6775の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab6775 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6775 は 5 報の論文で使用されています。

  • Guo Z  et al. IL-10 Promotes CXCL13 Expression in Macrophages Following Foot-and-Mouth Disease Virus Infection. Int J Mol Sci 24:N/A (2023). PubMed: 37047294
  • Freitas FB  et al. The QP509L and Q706L superfamily II RNA helicases of African swine fever virus are required for viral replication, having non-redundant activities. Emerg Microbes Infect 8:291-302 (2019). PubMed: 30866783
  • Freitas FB  et al. African swine fever virus encodes for an E2-ubiquitin conjugating enzyme that is mono- and di-ubiquitinated and required for viral replication cycle. Sci Rep 8:3471 (2018). PubMed: 29472632
  • Frouco G  et al. DNA-Binding Properties of African Swine Fever Virus pA104R, a Histone-Like Protein Involved in Viral Replication and Transcription. J Virol 91:N/A (2017). PubMed: 28381576
  • Simões M  et al. Alterations of Nuclear Architecture and Epigenetic Signatures during African Swine Fever Virus Infection. Viruses 7:4978-96 (2015). PubMed: 26389938

レビューと Q&A

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Question

Good afternoon,

we would like to ask for help regarding the next secondary antibodies, guiding me through the choice to use some antibodies for Immunofluorescence in combined forms.

The target cells are VERO, infected with a porcine virus.

Some of the primary antibodies have then to be tagged with a goat anti-mouse Cy3 or a donkey anti-mouse FITC.

To this primary anti-mouse marking we perform another signaling mark with a rabbit anti-pig (ab6775 - already in our lab) or one of the following items:

rabbit a-pig ab6773 (FITC)
goat a-pig ab6911 (FITC), Which should result better? Should we be careful about the Fc portion in such combinations?

To add up to this confusing situation we also have a need for a anti-rabbit Dylight 594. Should we prefer a donkey anti-rabbit (ab 96921 or 96893) or,
a goat anti-rabbit (ab 96885, 96897 or 98473)?

Could it be possible to endorse the Invoice proforma of the recommended anti-pig FITC and anti-rabbit Dylight 594 antibodies to:

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Answer

Thank you very much for your inquiry and your interest in our products.

If performing a double or triple staining, it is very important to make all the controls that the secondary antibodies do not crossreact with the immunoglobulins from the other species. For example for the anti pig antibody, it would be important that the anti pig antibody does not recognise the immunoglobulins from mouse or rabbit, not to have a false collocatisation. Unfortunately, non of our anti pig antibodies have been tested for this. However as you have used the ab6775 already successfully in your laboratory if I understood correctly, I can recommend to use the ab6773 as it is from the same original pool. Please do however perform the single color controls to verify the crossreactivity of the ab6773 with rabbit or mouse immunoglobulins.

In regards to the anti rabbit antibody, I can recommend to use the ab98500, a donkey anti rabbit IgG which has been preabsorbed against mouse, rat and human immunoglobulins, sothere is nocrossreaction against those immunoglobulins. We do not have an anti rabbit DyLight 594 which has been crossabsorbed also against the pig immunoglobulins. If you are concerned or if you have troubles with background staining on the VERO cells, I can recommend to use the same antibody , however with no Fc region, i.e. the F(ab')2 portion. This would be the ab98490.
https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-594-preadsorbed-ab98500.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-594-preadsorbed-ab98500.html).
https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLightreg-594-pre-adsorbed-ab98490.html (or use the following: https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLightreg-594-pre-adsorbed-ab98490.html).
Please let us know which one you would prefer, so we can send the correctproforma invoice.

The species in which the secondary antibody is generated is not very important. It is only important if you get background staining from the secondary antibody alone, in which case you can then block with serum of that species. However this is not an issue in general, especially not in cell culture.

I hope this information is helpful.

We will send theproforma invoices tomorrow also to your email address. I hope this is how you would like to receive it. Please let me know however whether you would like us to send it to another email address or fax.

Thank you very much. Please do not hesitate to contact us for any further question or advice.

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