Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
Key features and details
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: ICC/IF, IHC-P
製品の概要
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製品名
Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
IgG + IgM + IgA 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Mouse -
特異性
Antibodies were raised in rabbit to mouse IgG, IgM and IgA -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound. -
特記事項（標識）
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
ポリ/モノ
ポリクローナル -
特記事項
F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent dilution. PubMed: 15483223
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| IHC-P |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent dilution. PubMed: 15483223 |
|
IHC-P
Use at an assay dependent dilution. |
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab8517 は 13 報の論文で使用されています。
- Lahimer M et al. Paternal Age Matters: Association with Sperm Criteria's- Spermatozoa DNA Integrity and Methylation Profile. J Clin Med 12:N/A (2023). PubMed: 37568329
- Hajizadeh-Tafti F et al. Xeno-free generation of new Yazd human embryonic stem cell lines (Yazd4-7) as a prior stage toward good manufacturing practice of clinical-grade raw materials from discarded embryos: A lab resources report. Int J Reprod Biomed 21:619-628 (2023). PubMed: 37885973
- Zhao M et al. The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells. Cell Prolif 53:e12758 (2020). PubMed: 31922317
- Akyash F et al. Derivation of new human embryonic stem cell lines (Yazd1-3) and their vitrification using Cryotech and Cryowin tools: A lab resources report. Int J Reprod Biomed (Yazd) 17:891-906 (2019). PubMed: 31970311
- Kolodziej M et al. First molecular characterization of Dobrava-Belgrade virus found in Apodemus flavicollis in Poland. Ann Agric Environ Med 25:368-373 (2018). PubMed: 29936795