Rabbit Anti-Mouse IgG H&L (ab6709)
Key features and details
- Rabbit Anti-Mouse IgG H&L
- Host species: Rabbit
- Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
製品の概要
-
製品名
Rabbit Anti-Mouse IgG H&L
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Mouse -
アプリケーション
適用あり: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
免疫原
Mouse IgG, whole molecule
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
-
精製度
Affinity purified -
特記事項(精製)
Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. -
ポリ/モノ
ポリクローナル -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6709の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000.
|
|
IP |
Use at an assay dependent concentration.
|
|
Immunodiffusion |
Use at an assay dependent concentration.
|
|
Conjugation |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
ELISA |
1/20000 - 1/400000.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
1/2000 - 1/10000. |
IP
Use at an assay dependent concentration. |
Immunodiffusion
Use at an assay dependent concentration. |
Conjugation
Use at an assay dependent concentration. |
ICC/IF
1/1000 - 1/5000. |
ELISA
1/20000 - 1/400000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
-
Sensitivity of ab6709 against mouse IgG.
The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (52)
ab6709 は 52 報の論文で使用されています。
- Ai L et al. Tempol alleviates acute lung injury by affecting glutathione synthesis through Nrf2 and inhibiting ferroptosis in lung epithelial cells. J Biochem Mol Toxicol 38:e23674 (2024). PubMed: 38454815
- Li J et al. N6-methyladenosine-modified circSLCO1B3 promotes intrahepatic cholangiocarcinoma progression via regulating HOXC8 and PD-L1. J Exp Clin Cancer Res 43:119 (2024). PubMed: 38641828
- Read KA et al. Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity. Nat Commun 14:1652 (2023). PubMed: 36964178
- Qiu P et al. SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway. Can J Gastroenterol Hepatol 2023:2021515 (2023). PubMed: 36967718
- Wang K et al. Intercellular mitochondrial transfer alleviates pyroptosis in dental pulp damage. Cell Prolif 56:e13442 (2023). PubMed: 37086012