AB6727

Rabbit Anti-Mouse IgG H&L (Biotin)

Name: Rabbit Anti-Mouse IgG H&L (Biotin) (ab6727) | Abcam
Brand: Abcam Limited
SKU: AB6727
Price: 41000 JPY
Availability: InStock

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(4 Publications)

Rabbit Anti-Mouse IgG H&L (Biotin) (ab6727) is a biotin conjugated secondary antibody that is suitable for ELISA applications.

別名を表示する

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images

ELISA - Rabbit Anti-Mouse IgG H&L (Biotin) (AB6727)

ELISA

Supplier Data

ELISA - Rabbit Anti-Mouse IgG H&L (Biotin) (AB6727)

ELISA results of ab6727 against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (green line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin HRP conjugate 1/10,000 and TMB-8000 substrate.

Key facts

宿主種

Rabbit

ターゲット種

Mouse

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

血清吸着処理済み

標識

Biotin

励起波長/蛍光波長

アプリケーション

ELISA

applications

クローン性

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/300000", "notes":"<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Ighg1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

JOR spine 8:e70054 PubMed40061888

2025

Application and Validation of Semiautomatic Quantification of Immunohistochemically Stained Sections for Low Cellular Tissue Such as Intervertebral Disc Using QuPath.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Nüesch,Maria Paola Ferri,Christine L Le Maitre

Cells 13: PubMed39272974

2024

The Expression of Toll-like Receptors in Cartilage Endplate Cells: A Role of Toll-like Receptor 2 in Pro-Inflammatory and Pro-Catabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Mengis,Laura Bernhard,Andrea Nüesch,Irina Heggli,Nick Herger,Jan Devan,Roy Marcus,Christoph J Laux,Florian Brunner,Mazda Farshad,Oliver Distler,Christine L Le Maitre,Stefan Dudli

JOR spine 3:e1098 PubMed33015573

2020

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie Binch,Joseph Snuggs,Christine L Le Maitre

International journal of oncology 54:139-151 PubMed30431105

2018

HIF-1α contributes to tube malformation of human lymphatic endothelial cells by upregulating VEGFR-3.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Han,Jun Yan,Haini Chen,Yi Ji,Jianbing Chen,Jie Cui,Weimin Shen,Jijun Zou

View all publications

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Rabbit Anti-Mouse IgG H&L (Biotin)