Rabbit Anti-Human IgM mu chain (Alexa Fluor® 488) (ab150189)
Key features and details
- Rabbit Anti-Human IgM mu chain (Alexa Fluor® 488)
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Flow Cyt, IHC-P, ELISA, ICC/IF
Related conjugates and formulations
製品の概要
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製品名
Rabbit Anti-Human IgM mu chain (Alexa Fluor® 488)
IgM 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Human -
アプリケーション
適用あり: Flow Cyt, IHC-P, ELISA, ICC/IFmore details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150189の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/2000.
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| 特記事項 |
|---|
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Flow Cyt
1/2000. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Human IgM mu chain (Alexa Fluor® 488) (ab150189)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human Normal Tonsil labelled for Human IgM mu chain using ab150189. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab150189 (Rabbit Anti-Human IgM mu chain (Alexa Fluor® 488) at 1/2000 dilution. and counterstained using ab195889 (Alexa Fluor® 594 Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker) at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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Alexa Fluor® - Rabbit Anti-Human IgM mu chain (Alexa Fluor® 488) (ab150189)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab150189 は論文での使用が確認できていません。