Rabbit Anti-Human IgM H&L (FITC) (ab8506)
Key features and details
- Rabbit Anti-Human IgM H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: ICC/IF
Related conjugates and formulations
製品の概要
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製品名
Rabbit Anti-Human IgM H&L (FITC)
IgM 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Human -
アプリケーション
適用あり: ICC/IFmore details -
標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound. -
特記事項（標識）
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
ポリ/モノ
ポリクローナル -
特記事項
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgM is depostited. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8506の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
| 特記事項 |
|---|
追加情報
For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab8506 は 1 報の論文で使用されています。
- Takegaki J et al. Influence of shortened recovery between resistance exercise sessions on muscle-hypertrophic effect in rat skeletal muscle. Physiol Rep 7:e14155 (2019). PubMed: 31250976