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Rabbit Anti-Goat IgG+IgM+IgA H&L (HRP) (ab8515)

Submit a review Q&A (1)References (1)

Key features and details

  • Rabbit Anti-Goat IgG+IgM+IgA H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ELISA, Dot blot

製品の概要

  • 製品名

    Rabbit Anti-Goat IgG+IgM+IgA H&L (HRP)
    IgG + IgM + IgA 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Goat

  • 特異性

    Antibodies were raised in rabbit to goat IgG, IgM and IgA

  • アプリケーション

    適用あり: ELISA, Dot blotmore details

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Specific antibodies were absorpted by incubation with Sepharose-bound goat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized goat immunoglobulins a minimum of 65% protein bound.

  • 特記事項（標識）

    The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8515の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/1000.
Dot blot
1/100.
特記事項
ELISA
1/1000.
Dot blot
1/100.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab8515 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8515 は 1 報の論文で使用されています。

  • Wall VZ  et al. Inflammatory stimuli induce acyl-CoA thioesterase 7 and remodeling of phospholipids containing unsaturated long (=C20)-acyl chains in macrophages. J Lipid Res 58:1174-1185 (2017). PubMed: 28416579

レビューと Q&A

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レビューを送る 質問を送る

Question

Need to checked with you if it is possible to use ab8515 (anti-gt IgG+IgM+IgA) to measure only the IgA level from lung lavage fluid secretion in goat since I could not find any secondary for anti-goat IgA? Will the final absorbance value be inaccurate?

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Answer

Unfortunately, ab8515 will not be suitable to measure IgA levels as it also recognizes at the same time IgG+IgM. We do not have any antibodies against goat IgA- only for mouse, rabbit and human IgAs.

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