Rabbit Anti-Goat IgG H&L (HRP)

• WB

Supplier Data

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (AB6741)

ab6741 was used at dilution 1/5000 with the primary antibody ab23938 in WB. See the review on ab23938.

Secondary

All lanes:

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution

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• WB

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Western blot - Rabbit Anti-Goat IgG H&L (HRP) (AB6741)

Western blot of EMSY on MCF-7 cell lysate.

Lane 1 : ab4579 at 1/500
Lane 2 : ab123 at 1/500.

Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.

Secondary ab Lane 1 : Rabbit polyclonal to Goat IgG H&L (HRP) ab6741 (1/5000)
Exposure time : 3 min.

Secondary ab Lane 2 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
Exposure time : 10 sec.

Lysate at 20μg/lane.

Lane 1 - Exposure time : 3 min.
Lane 2 - Exposure time : 10 sec.

Variability in the size at which EMSY runs by Western blot has been exper

Lane 1:

Anti-EMSY antibody (ab4579) at 1/500 dilution

Lane 2:

Western blot - Anti-EMSY antibody (<a href='/products/primary-antibodies/emsy-antibody-ab123'>ab123</a>) at 1/500 dilution

All lanes:

MCF-7 cell lysate at 20 µg

Secondary

Lane 1:

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab7090'>ab7090</a>) at 1/5000 dilution

Predicted band size: 141 kDa

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• ELISA

AbReview19545****

ELISA - Rabbit Anti-Goat IgG H&L (HRP) (AB6741)

ab6741 was used at dilution 1/1000 with the primary antibody ab2247 in ELISA. See the review on ab2247.

This image is courtesy of an anonymous Abreview.

• ELISA

Supplier Data

ELISA - Rabbit Anti-Goat IgG H&L (HRP) (AB6741)

ELISA results of ab6741 against purified Goat IgG protein. Each well was coated in duplicate with 1.0 µg of Goat IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/22,700. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.

• WB

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Western blot - Rabbit Anti-Goat IgG H&L (HRP) (AB6741)

<ab12079>ab12079>Histone H3 antibody (ab12079) at 0.5 ug/ml, HeLa Cell Histone prep at 5 ug

Secondary
Rabbit polyclonal to Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 15kD

All lanes:

Anti-Histone H3 antibody - ChIP Grade (<a href='/products/unavailable/histone-h3-antibody-chip-grade-ab12079'>ab12079</a>) at 0.5 µg/mL

All lanes:

HeLa Cell Histone prep at 5 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution

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• WB

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Western blot - Rabbit Anti-Goat IgG H&L (HRP) (AB6741)

Secondary antibody - rabbit anti-goat HRP (ab6741)

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/products/primary-antibodies/beta-actin-antibody-loading-control-ab8229'>ab8229</a>) at 1/1000 dilution

Lane 1:

HeLa whole cell (Human) at 20 µg

Lane 2:

3T3 cell (Mouse) at 20 µg

Lane 3:

Rabbit Liver at 20 µg

Lane 4:

MDCK cell (Dog) at 20 µg

Lane 5:

EBTr cell (Cow) at 20 µg

Lane 6:

SL-29 cell (Chicken) at 20 µg

Lane 7:

CHO cell (Chinese Hamster) at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 42 kDa

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Exposure time: 5min

• WB

Supplier Data

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (AB6741)

Western blot analysis of ab6741 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Goat IgG at 100 ng

Secondary

All lanes:

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/1000 dilution

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