Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab169345)
Key features and details
- Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488)
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Host species: Rabbit
- Suitable for: IHC-Fr, Flow Cyt, IHC-P, ELISA, ICC/IF
Related conjugates and formulations
製品の概要
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製品名
Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Mouse -
アプリケーション
適用あり: IHC-Fr, Flow Cyt, IHC-P, ELISA, ICC/IFmore details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. -
ポリ/モノ
ポリクローナル -
特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169345の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.
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| IHC-P |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| 特記事項 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.
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IHC-P
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
画像
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Immunocytochemistry/ Immunofluorescence - Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab169345)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab169345, Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (green) was then used at 1µg/ml for 1h at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab169345)HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab169345, Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (green) was used at 1µg/ml for 1h at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Alexa Fluor® - Rabbit F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab169345)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab169345 は 2 報の論文で使用されています。
- Kim HY et al. RAGE-specific single chain Fv for PET imaging of pancreatic cancer. PLoS One 13:e0192821 (2018). PubMed: 29529089
- Yang Z et al. Inhibition of p38 MAPK Signaling Regulates the Expression of EAAT2 in the Brains of Epileptic Rats. Front Neurol 9:925 (2018). PubMed: 30429824