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Mouse monoclonal [MARE-1] Anti-Rat IgE heavy chain (Biotin) (ab11666)

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Key features and details

  • Mouse monoclonal [MARE-1] Anti-Rat IgE heavy chain (Biotin)
  • Conjugation: Biotin
  • Host species: Mouse
  • Isotype: IgG1
  • Suitable for: WB, ELISA

製品の概要

  • 製品名

    Mouse monoclonal [MARE-1] Anti-Rat IgE heavy chain (Biotin)
    IgE 二次抗体 製品一覧

  • 由来種

    Mouse

  • ターゲット生物種

    Rat

  • 特異性

    This antibody is specific for the epsilon chain of rat IgE and does not cross-react with other classes of rat immunoglobulin.

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 免疫原

    Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Purified IgG prepared by affinity chromatography on IR2 rat IgE myeloma.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MARE-1

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG1

  • 特記事項

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11666の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent dilution. Predicted molecular weight: 51.4 kDa.
ELISA
Use a concentration of 0.5 µg/ml.
特記事項
WB
Use at an assay dependent dilution. Predicted molecular weight: 51.4 kDa.
ELISA
Use a concentration of 0.5 µg/ml.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab11666 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11666 は論文での使用が確認できていません。

レビューと Q&A

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Question



Inquiry: iI want to perform westren blotting using ab11666, i already have this product .
i want to know if i can use directly ab 7239 which is rabbit polyclonal to steptavidine (HRP) or do i need to label it with steptavidine first and then i shall go for its development using ab7239
what do u suggest if i have to use ab11666 as secondary antibody and have to develop it with DAB or chemiluminescent method

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Answer

Thank you for contacting us.


I am not sure if I understood correctly:

You want to perform a WB with ab11666 as the secondary antibody, you will need the following:

a) primary antibody, host animal rat

b) Streptavidin conjugated with HRP to get a chemoluminescence signal caught on film with a suitable substrate or

c) use DAB for a chromogenic reaction (which is far less sensitiv than chemoluminescence )

Unfortunately, I don't quite understand what you are planning with the ab7239. This is an antibody made in rabbits, so the ab11666 will not react with it.


I hope this information is helpful to you. Please let me know if I misunderstood you.

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