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Mouse monoclonal [5A9] Anti-Human IgG Fc (HRP) (ab7499)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [5A9] Anti-Human IgG Fc (HRP)
  • Conjugation: HRP
  • Host species: Mouse
  • Isotype: IgG2a
  • Suitable for: Sandwich ELISA, ELISA

製品の概要

  • 製品名

    Mouse monoclonal [5A9] Anti-Human IgG Fc (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Mouse

  • ターゲット生物種

    Human

  • アプリケーション

    適用あり: Sandwich ELISA, ELISAmore details

  • 免疫原

    These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with IgG from human serum.

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.40
    Preservative: 0.05% Proclin 300
    Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    5A9

  • アイソタイプ

    IgG2a

  • 特記事項

    Concentration varies from lot to lot and can be provided on request.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7499の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
特記事項
Sandwich ELISA
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab7499 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7499 は 3 報の論文で使用されています。

  • Cheng YJ  et al. The characters of antibodies against PLA2R in healthy individuals and in the patient with PLA2R associated membranous nephropathy. Eur J Med Res 28:128 (2023). PubMed: 36935517
  • Padula L  et al. Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responses. Vaccine X 12:100202 (2022). PubMed: 35936992
  • Mboumba Bouassa RS  et al. Serum and cervicovaginal IgG immune responses against a7 and a9 HPV in non-vaccinated women at risk for cervical cancer: Implication for catch-up prophylactic HPV vaccination. PLoS One 15:e0233084 (2020). PubMed: 32421735

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

This secondary is cross-reacting with denatured BSA. Previous lots have not been like this & FOCR also did the same.

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Answer

Thank you for calling Abcam earlier today.

I am sorry that the first replacement antibody we sent you did not prove to be successful and so as we discussed I am sending you a vial of ab99765. Your order number, for your records for this product is *-****.

If there is anything else I can help you with, please let me know.

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Question

This secondary is cross-reacting with denatured BSA. Previous lots have not been like this.

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement order.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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