Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
Key features and details
- Goat Anti-Rat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blot
製品の概要
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製品名
Goat Anti-Rat IgG H&L (HRP) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
特異性
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
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アプリケーション
適用あり: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blotmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
免疫原
Full length native Rat IgG (purified).
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Horseradish Peroxidase (HRP) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500 - 1/1500.
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IHC-P |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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|
WB |
Use at an assay dependent dilution.
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|
ELISA |
1/50000.
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|
Dot blot |
Use at an assay dependent dilution.
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特記事項 |
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ICC/IF
1/500 - 1/1500. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. |
ELISA
1/50000. |
Dot blot
Use at an assay dependent dilution. |
画像
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Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097) at 1/1000 dilution + Rat IgG, 50 ng
Both the heavy and light chains of rat IgG are detected.
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IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)This picture was kindly supplied as part of the review submitted by Helen Makarenkova.
Whole mount immunostaining of E11.5 mouse embryo (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab7097 (HRP conjugated).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (57)
ab7097 は 57 報の論文で使用されています。
- Zhang M et al. Anti‑silencing function 1B promotes the progression of pancreatic cancer by activating c‑Myc. Int J Oncol 62:N/A (2023). PubMed: 36416310
- Mudgal R et al. The neuroprotective effect of ascorbic acid against imidacloprid-induced neurotoxicity and the role of HO-1 in mice. Front Neurol 14:1130575 (2023). PubMed: 37153653
- Xu X et al. Salvianolic Acid B Inhibits Ferroptosis and Apoptosis during Myocardial Ischemia/Reperfusion Injury via Decreasing the Ubiquitin-Proteasome Degradation of GPX4 and the ROS-JNK/MAPK Pathways. Molecules 28:N/A (2023). PubMed: 37241859
- Pereira J et al. Gene identification and RNAi-silencing of p62/SQSTM1 in the vector Rhodnius prolixus reveals a high degree of sequence conservation but no apparent deficiency-related phenotypes in vitellogenic females. PLoS One 18:e0287488 (2023). PubMed: 37486954
- Ren X et al. Astragalus polysaccharide restores insulin secretion impaired by lipopolysaccharides through the protein kinase B /mammalian target of rapamycin/glucose transporter 2 pathway. BMC Complement Med Ther 23:358 (2023). PubMed: 37817130