Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846)
Key features and details
- Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
Related conjugates and formulations
Alexa Fluor® 405
Alexa Fluor® 488
Alexa Fluor® 555
Alexa Fluor® 568
Alexa Fluor® 594
Alexa Fluor® 647
Alexa Fluor® 680
Alexa Fluor® 750
Alexa Fluor® 790
Biotin
Cy2 ®
Cy3 ®
Cy5 ®
Cy5.5 ®
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 650
FITC
Gold 10nm
HRP
HRP polymer
PE
PE
Texas Red ®
TRITC
Unconjugated
Unconjugated
製品の概要
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製品名
Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
アプリケーション
適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rat IgG whole molecule
-
標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6846の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/2500.
|
|
ELISA |
1/15000.
|
|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/200 - 1/1000.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
1/500 - 1/2500. |
ELISA
1/15000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
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All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/5000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
Lanes 3-4 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
Lanes 5-6 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/50000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
Developed using the ECL technique.
Performed under reducing conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab6846 は 4 報の論文で使用されています。
- Morishige JI et al. Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes. J Lipid Res 64:100450 (2023). PubMed: 37751791
- Mukai K et al. Topical estrogen application promotes cutaneous wound healing in db/db female mice with type 2 diabetes. PLoS One 17:e0264572 (2022). PubMed: 35271602
- Stromberg LR et al. Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling. PLoS One 13:e0198531 (2018). PubMed: 29902192
- Tarver MR et al. Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus). Insects 3:1190-9 (2012). PubMed: 26466734