Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Key features and details
- Goat Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.80
Constituents: 0.2% BSA, PBS, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
関連製品
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Alternative Versions
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Related Products
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Substrate reagent
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- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97051の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000.
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WB | (10) |
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000
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特記事項 |
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ICC
Use at an assay dependent concentration. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. |
WB
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000 |
画像
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All lanes : Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) at 1/1000 dilution
Lane 1 : Rat pituitary whole tissue lysate
Lane 2 : Mouse pituitary whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilutionExposure time: 1st lane: 85 seconds
2nd lane: 32 secondsBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
Lane 2 : T-47D (human mammary gland ductal carcinoma epithelial cell). Whole cell lysates
Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 5 : Human uterus whole tissue lysate
Lane 6 : Human ovary whole tissue lysate
Lane 7 : Human ovary cancer whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Exposure time: 50 secondsBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab32063 at a working dilution of 1 in 200. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Western blot analysis of co-expression Bacillus anthracis PA83 (A), Pfs48/45 (B) and Pfs48/45-10C with bacterial Endo H or PNGase F in N. benthamiana plants.
(A) Western blot analysis of co-expression of PA83. Lanes: 1- N. benthamiana plant was infiltrated with pBI-PA83 construct, for the production of glycosylated PA83, 2,3- N. benthamiana plants were infiltrated with combinations of the pBI-Endo H/pBI-PA83 or pBI-PNGase F/pBI-PA83 constructs, for the production of Endo H (2) or PNGase F (3) deglycosylated PA83 proteins.
(B) Western blot analysis of co-expression of Pfs48/45. Lanes: 1-N. benthamiana plant was infiltrated with pEAQ-Pfs48/45 construct for the production of glycosylated Pfs48/45;2,3- N. benthamiana plants were infiltrated with combinations of the pBI-Endo H/pEAQ-Pfs48/45 or pBI-PNGase F/pEAQ-Pfs48/45constructs for the production of Endo H (2) and PNGase F (3) deglycosylated Pfs48/45 proteins.
(C) Western blot analysis of co-expression of Pfs48/45-10C. Lanes: 1- N. benthamiana plant was infiltrated with pEAQ-Pfs48/45-10C construct for the production of glycosylated Pfs48/45-10C; 2,3- N. benthamiana plants were infiltrated with combinations of the pBI-Endo H/pEAQ-Pfs48/45 or pBI-PNGase F/pEAQ-Pfs48/45constructs for the production of Endo H (2) and PNGase F (3) deglycosylated Pfs48/45-10C proteins. gPA83- glycosylated PA83; dPA83- deglycosylated PA83; gPfs48/45: glycosylated Pfs48/45; dPfs48/45: deglycosylated Pfs48/45; gPfs48/45-10C: glycosylated Pfs48/45-10C; dPfs48/45-10C: deglycosylated Pfs48/45-10C.
M: MagicMark XP Western Protein Standard. PA83 proteins were detected using the anti-Bacillus anthracis protective antigen antibody BAP0101 (Cat. No. ab1988, Abcam); Ps48/45, Endo H or PNGase F proteins were detected using the anti-FLAG antibody. Pfs48/45-10C protein was detected using the purified anti-His Tag antibody.
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IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30mins. The section was incubated with ab8227, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Anti-LAMP2 antibody (ab37024) at 1/1000 dilution + Mouse brain whole tissue lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute10 % gel. Blocked with 5% BSA for 2 hours at 25°C.
Incubated with the primary antibody for 1 hour in TBS-tween at 25°C.
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All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Rabbit polyclonal to GNAT2 (ab97501) at 1/2000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1097)
ab97051 は 1097 報の論文で使用されています。
- Reis-Mendes A et al. The Role of Nrf2 and Inflammation on the Dissimilar Cardiotoxicity of Doxorubicin in Two-Time Points: a Cardio-Oncology In Vivo Study Through Time. Inflammation 47:264-284 (2024). PubMed: 37833616
- Li S et al. Transcriptionally activates CCL28 expression to inhibit M2 polarization of macrophages and prevent immune escape in colorectal cancer cells. Transl Oncol 40:101842 (2024). PubMed: 38035446
- Wang Q et al. microRNA profilings identify plasma biomarkers and targets associated with pediatric epilepsy patients. Pediatr Res 95:996-1008 (2024). PubMed: 37884644
- Zhou R et al. Silencing of GDF11 suppresses hepatocyte apoptosis to relieve LPS/D-GalN acute liver failure. J Biochem Mol Toxicol 38:e23577 (2024). PubMed: 37934488
- Yang Y et al. Brucine D restrains colorectal cancer tumorigenesis and autophagy by downregulating circ_0068464. Chem Biol Drug Des 103:e14407 (2024). PubMed: 38040413