Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
Key features and details
- Goat Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Frmore details -
免疫原
Rabbit IgG, whole molecule
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. -
特記事項(標識)
Horseradish Peroxidase (HRP) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. -
研究分野
関連製品
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Related Products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6721の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (2) |
1/1000.
|
WB | (10) |
1/2000 - 1/20000.
Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283). Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances. |
ELISA |
1/120000.
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|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ICC |
1/1000 - 1/5000.
|
|
IHC-Fr |
1/1000.
|
特記事項 |
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IHC-P
1/1000. |
WB
1/2000 - 1/20000. Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283). Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances. |
ELISA
1/120000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC
1/1000 - 1/5000. |
IHC-Fr
1/1000. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)Farah et al PLoS One. 2018 Feb 2;13(2):e0191526. doi: 10.1371/journal.pone.0191526. eCollection 2018. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Anti-phospho-tau immunostaining in Dryocopus lineatus.
Tau-positivity in the midbrain (Panel A (shown) and B) and the corpus callosum (C) of the Dryocopus lineatus brain. The axonal tract staining demonstrates a thread-like pattern, similar to that seen with Gallyas sliver staining. Occasional intracellular tau-accumulations were identified within neurons (D).
For full method, please see paper.
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Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)This image is courtesy of an Abreview submitted by Maryna Polyakova.All lanes : Anti-NEK7 antibody (ab80948) at 1/2000 dilution
All lanes : Mouse brain tissue cytoplasmic lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 secondsBlocked with 5% non-fat milk for 1 hour at 18°C
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)This image is courtesy of an anonymous Abreview.
ab3580 staining glucocorticoid receptor in mouse epididymis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with Bouin's solution and blocked with 1.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 14 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab6721) (1/200) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)Image is courtesy of an anonymous AbReview.
Immunohistochemical analysis of PFA-fixed paraffin-embedded rat cardiac tissue sections, labeling Conexin 43 with ab117843 at a dilution of 1/500 incubated for 12 hours at 4°C in 1% BSA in TBS. Antigen retrival was via Tris-EDTA pH 9.0 (heat mediated). Blocking was 3% BSA incubated for 1 hour at 37°C. The secondary was ab6721 at 1/500.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5942)
ab6721 は 5942 報の論文で使用されています。
- Xu Z et al. Epigenetic upregulation of MNAT1 by SMYD2 is linked to PI3K/AKT activation and tumorigenesis of pancreatic adenocarcinoma. Histol Histopathol 39:263-277 (2024). PubMed: 37232506
- Qiao W et al. Identification of potential hub genes associated with atopic dermatitis-like recombinant human epidermal model using integrated transcriptomic and proteomic analysis. Biomol Biomed 24:89-100 (2024). PubMed: 37540585
- Fan M et al. IRE1α regulates the PTHrP-IHH feedback loop to orchestrate chondrocyte hypertrophy and cartilage mineralization. Genes Dis 11:464-478 (2024). PubMed: 37588212
- Bi Y et al. A novel FGFR1 inhibitor CYY292 suppresses tumor progression, invasion, and metastasis of glioblastoma by inhibiting the Akt/GSK3β/snail signaling axis. Genes Dis 11:479-494 (2024). PubMed: 37588207
- Chen J et al. MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression. Environ Toxicol 39:357-366 (2024). PubMed: 37755144