Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Rabbit IgG, whole molecule
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標識
Cy5 ®. Ex: 650nm, Em: 667nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
特記事項(標識)
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6564の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | (1) |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/5000.
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ELISA |
1/10000 - 1/50000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
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Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)Hennenberg, M. et al PLoS One. 2012;7(11):e50904. doi: 10.1371/journal.pone.0050904. Epub 2012 Nov 30 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunofluorescence stainings of human prostate tissues
Sections were double labeled for different PAK isoforms, together with calponin (marker for smooth muscle cells), pan-cytokeratin (marker for epithelial cells of glands), or tyrosine hydroxylase (TH, marker for catecholaminergic nerves). Yellow color in merged pictures may indicate colocalization of targets. Shown are representative stainings from series with tissues from n = 5 patients for each combination.
ab6908 was used as secondary antibody to visualize various rabbit anti-PAK primary antibodies (red).
(From Figure 2 of Hennenberg et al)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)This image is courtesy of an anonymous Abreview.
ab11175 staining Histone H2A.X in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 4% BSA for 2 hours at 37°C. Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS/4% BSA/0.05% Triton X-100) for 48 hours at 4°C. A Cy5®-conjugated goat anti-rabbit IgG H&L preadsorbed (ab6564) (1/300) was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (82)
ab6564 は 82 報の論文で使用されています。
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- Su VY et al. The main e-cigarette component vegetable glycerin enhances neutrophil migration and fibrosis in endotoxin-induced lung injury via p38 MAPK activation. Respir Res 24:9 (2023). PubMed: 36627690
- Mysiewicz S et al. Interspecies and regional variability of alcohol action on large cerebral arteries: regulation by KCNMB1 proteins. Am J Physiol Regul Integr Comp Physiol 324:R480-R496 (2023). PubMed: 36717168