Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab7089)
Key features and details
- Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Goat
- Suitable for: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Fr
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
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アプリケーション
適用あり: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Frmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Full length native Rabbit IgG (purified).
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
ポリ/モノ
ポリクローナル -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7089の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | ||
| WB | ||
| ELISA | ||
| Immunomicroscopy | ||
| Dot blot | ||
| IHC-P | ||
| IHC-Fr |
| 特記事項 |
|---|
追加情報
Dot: Use at an assay dependant dilution.
ELISA: 1/10000 - 1/40000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/10000 - 1/40000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab7089 は 6 報の論文で使用されています。
- Veggiani G et al. Panel of Engineered Ubiquitin Variants Targeting the Family of Human Ubiquitin Interacting Motifs. ACS Chem Biol 17:941-956 (2022). PubMed: 35385646
- Ling B et al. Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D. Oncol Rep 45:1044-1058 (2021). PubMed: 33650655
- Xu M et al. Blockage of C-X-C Motif Chemokine Receptor 2 (CXCR2) Suppressed Uric Acid (UA)-Induced Cardiac Remodeling. Front Physiol 12:700338 (2021). PubMed: 34385934
- Guan X et al. Long non-coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression. Oncol Rep 44:2465-2474 (2020). PubMed: 33125146
- Kim DH et al. Direct visualization of single-molecule membrane protein interactions in living cells. PLoS Biol 16:e2006660 (2018). PubMed: 30543635
- Tan JY et al. Cross-talking noncoding RNAs contribute to cell-specific neurodegeneration in SCA7. Nat Struct Mol Biol 21:955-61 (2014). WB . PubMed: 25306109
