Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Key features and details
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICC
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICCmore details -
免疫原
Rabbit IgG whole molecule
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標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Anti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6722の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/4000.
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ELISA |
1/3000 - 1/25000.
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Dot blot |
Use at an assay dependent concentration.
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|
ICC/IF |
1/200 - 1/1000.
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IHC-Fr |
Use at an assay dependent concentration.
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|
ICC |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000 - 1/4000. |
ELISA
1/3000 - 1/25000. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
IHC-Fr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
画像
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Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) Fragment.
Lane 3: Rabbit IgG F(c) Fragment.
Lane 4: Rabbit IgM Whole Molecule.
Lane 5: Normal Rabbit Serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
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Dot Blot of ab6722.
Antigen: Rabbit IgG.
Load: Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
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Lane 1: Rabbit IgG.
Load: 100 ng per lane.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (51)
ab6722 は 51 報の論文で使用されています。
- Anttila K et al. Cardiovascular oxygen transport and peripheral oxygen extraction capacity contribute to acute heat tolerance in European seabass. Comp Biochem Physiol A Mol Integr Physiol 275:111340 (2023). PubMed: 36347467
- Chini A et al. Novel long non-coding RNAs associated with inflammation and macrophage activation in human. Sci Rep 13:4036 (2023). PubMed: 36899011
- Mazzaracchio V et al. Duplex Electrochemical Microfluidic Sensor for COVID-19 Antibody Detection: Natural versus Vaccine-Induced Humoral Response. Small 19:e2207731 (2023). PubMed: 36916701
- Dindi UMR et al. Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines. Front Pharmacol 14:1335305 (2023). PubMed: 38235110
- Napso T et al. Diet-induced maternal obesity impacts feto-placental growth and induces sex-specific alterations in placental morphology, mitochondrial bioenergetics, dynamics, lipid metabolism and oxidative stress in mice. Acta Physiol (Oxf) 234:e13795 (2022). PubMed: 35114078