AB7085

Goat Anti-Rabbit IgG H&L preadsorbed

Name: Goat Anti-Rabbit IgG H&L preadsorbed (ab7085) | Abcam
Brand: Abcam Limited
SKU: AB7085
Price: 36000 JPY
Availability: InStock

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(7 Publications)

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF, SDS-PAGE. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)

Western blot analysis of ab7085. Secondary antibody : Anti-Goat IgG (Donkey) Peroxidase Conjugated Antibody at 1/40000 dilution for 30 min at RT.

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (ab7085) at 1/1000 dilution

Lane 1:

Rabbit IgG at 50 ng

Lane 2:

Rabbit IgG F(ab) Fragment at 50 ng

Lane 3:

Rabbit IgG F(c) Fragment at 50 ng

Lane 4:

Rabbit IgM Whole Molecule at 50 ng

Lane 5:

Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (Goat) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. at 50 ng

Secondary

All lanes:

Anti-Goat IgG at 1/40000 dilution

false

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)

Western blot analysis of ab7085. Secondary antibody : DyLight™ 800 Goat secondary antibody 1/20000 dilution for 30 min at RT.

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (ab7085) at 1/1000 dilution

All lanes:

Rabbit IgG at 100 ng

Secondary

All lanes:

DyLight™ 800 goat secondary antibody 1:20,000 for 30 min at 1/20000 dilution

false

SDS-PAGE - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)

SDS-PAGE

Supplier Data

SDS-PAGE - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)

SDS-PAGE analysis of ab7085.

Lane 1 : Nonreduced Rabbit IgG whole molecule.

Lane 2 : Reduced Rabbit IgG whole molecule.

4-20% SDS Gel, Coomassie Blue Stained.

Key facts

宿主種

Goat

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

血清吸着処理済み

Yes

アプリケーション

ELISA, IP, IHC-Fr, ICC/IF, ID, IHC-P, SDS-PAGE, Conjugation, WB

applications

クローン性

Polyclonal

特異性

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA against at less than 1% of target signal.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/270000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2000", "notes":"" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9337 PubMed39472435

2024

Alternations in inflammatory macrophage niche drive phenotypic and functional plasticity of Kupffer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Ying Huang,Yan-Zhou Chen,Chuang Zhao,Xin-Nan Zheng,Kai Yu,Jia-Xing Yue,Huai-Qiang Ju,Yan-Xia Shi,Lin Tian

Cell reports 43:114406 PubMed38963759

2024

ALDH1A3-acetaldehyde metabolism potentiates transcriptional heterogeneity in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Lu,Jana Travnickova,Mihaly Badonyi,Florian Rambow,Andrea Coates,Zaid Khan,Jair Marques,Laura C Murphy,Pablo Garcia-Martinez,Richard Marais,Pakavarin Louphrasitthiphol,Alex H Y Chan,Christopher J Schofield,Alex von Kriegsheim,Joseph A Marsh,Valeria Pavet,Owen J Sansom,Robert S Illingworth,E Elizabeth Patton

Heliyon 9:e15872 PubMed37223716

2023

Efficacy of puerarin in rats with focal cerebral ischemia through modulation of the SIRT1/HIF-1α/VEGF signaling pathway and its effect on synaptic plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liu,Xiwen Sui,Yuqin Zhang,Rongchao Yue,Shifu Yin

RSC advances 12:31711-31728 PubMed36380932

2022

Nanotechnology meets immunology towards a rapid diagnosis solution: the COVID-19 outbreak challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Santos B S A S,Cunha J L R,Carvalho I C,Costa J M C,Longo B C,Galinari G C F,Diniz P H S M,Mendes G M M,Fonseca F G,Abrahão J S,Mansur A A P,Leite M F,Oréfice R L,Lobato Z I P,Mansur H S

Disease markers 2022:4607966 PubMed36267458

2022

Ferulic Acid Mitigates Growth and Invasion of Esophageal Squamous Cell Carcinoma through Inducing Ferroptotic Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Cao,Hong Zhang,Jianming Tang,Rui Wang

Oncology reports 40:241-251 PubMed29749472

2018

Insufficient radiofrequency ablation promotes hepatocellular carcinoma cell progression via autophagy and the CD133 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofei Wang,Qingsong Deng,Kai Feng,Shihan Chen,Jiayun Jiang,Feng Xia,Kuansheng Ma,Ping Bie

Molecular medicine reports 12:6738-44 PubMed26328522

2015

STAT3‑regulated long non‑coding RNAs lnc‑7SK and lnc‑IGF2‑AS promote hepatitis C virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Xiong,Ming Jia,Jing Yuan,Changjiang Zhang,Yan Zhu,Xuemei Kuang,Lin Lan,Xiaohong Wang

View all publications

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Goat Anti-Rabbit IgG H&L preadsorbed