Goat Anti-Rabbit IgG H&L (HRP)

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (<a href='/products/primary-antibodies/rab29-phospho-t71-antibody-mjf-r24-17-1-ab241062'>ab241062</a>) at 1/5000 dilution

Lane 1:

HEK-293 (human embryonic kidney) over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 15 µg

Lane 2:

MLi-2 (100nM for 60min) treated HEK-293 over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Histone H3 (mono methyl K79) with ab177183 at 1/5000 dilution, followed by ab97051 Goat Anti-Rabbit HRP (IgG H&L) at 1/500 dilution. Nucleus staining on Human skin tissue is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab32063 at a working dilution of 1 in 200. The secondary antibody used is ab97051 a HRP goat anti-rabbit IgG (H+L) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

ab92511 staining HLA-DR in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1 : PBS in place of primary antibody.

• IP

Supplier Data

Immunoprecipitation - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

DDR1 (phospho Y796) + DDR2 (phospho Y740) was immunoprecipitated from 0.35 mg T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate with ab322921 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322921 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (<a href='/products/primary-antibodies/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-ab322921'>ab322921</a>) at 1/1000 dilution

Lane 1:

T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-ab322921'>ab322921</a> at 1/30 IP in T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-ab322921'>ab322921</a> in T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

Observed band size: 125 kDa

false

Exposure time: 76s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL1r1-KO homozygous mice (Strain ID : T005977).

All lanes:

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (<a href='/products/primary-antibodies/il1-receptor-i-il-1r-1-antibody-epr22198-36-ab229051'>ab229051</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse spleen tissue lysate (female) at 20 µg

Lane 2:

IL1r1 knockout mouse spleen tissue lysate (female) at 20 µg

Lane 3:

Wild-type mouse spleen tissue lysate (male) at 20 µg

Lane 4:

IL1r1 knockout mouse spleen tissue lysate (male) at 20 µg

Lane 5:

Wild-type mouse thymus tissue lysate (female) at 20 µg

Lane 6:

IL1r1 knockout mouse thymus tissue lysate (female) at 20 µg

Lane 7:

Wild-type mouse thymus tissue lysate (male) at 20 µg

Lane 8:

IL1r1 knockout mouse thymus tissue lysate (male) at 20 µg

Secondary

All lanes:

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 81 kDa,36 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : IMR-90

The identity of the lower MW band at approximately 150 kDa (in lane 3 and lane4 ) is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-TRPM7 antibody [EPR27067-52] (<a href='/products/primary-antibodies/trpm7-antibody-epr27067-52-ab323270'>ab323270</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HEK-293 transfected with siRNA specifically targeting human TRPM7 whole cell lysate at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

HEK-293 whole cell lysate at 20 µg

Lane 5:

IMR-90 (human lung fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 250 kDa,124 kDa

false

Exposure time: 103s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands lower than 180 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR29645-145] (<a href='/products/primary-antibodies/werners-syndrome-helicase-wrn-antibody-epr29645-145-ab327707'>ab327707</a>) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human TRPM6

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TRPM7 antibody [EPR27067-52] (<a href='/products/primary-antibodies/trpm7-antibody-epr27067-52-ab323270'>ab323270</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TRPM7 expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TRPM6 expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 250 kDa,124 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : IMR-32, 293T ( PMID : 27383986).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-GSDMD antibody [EPR23078-13R] (<a href='/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a>) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 3:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 52 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Low/negative samples : SW480 (PMID : 31324782), HeLa (PMID : 1463733).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Prothrombin antibody [EPR20159] (<a href='/products/primary-antibodies/prothrombin-antibody-epr20159-ab208589'>ab208589</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 72 kDa,37 kDa

false

Exposure time: 20s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human EMP1 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (20-50KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-EMP2 antibody [EPR30305-511] (<a href='/products/primary-antibodies/emp2-antibody-epr30305-511-ab326423'>ab326423</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human EMP1 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human EMP2 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 20-50 kDa,36 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Raji, Jurkat, Daudi.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time :
Lane 1 : 81 seconds;
Lanes 2-6 : 180 seconds.

All lanes:

Western blot - Anti-EMP2 antibody [EPR30305-511] (<a href='/products/primary-antibodies/emp2-antibody-epr30305-511-ab326423'>ab326423</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Untreated SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SK-BR-3 whole cell lysate treated with Protein Deglycosylation MIX II at 20 µg

Lane 4:

Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 5:

Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 6:

Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 20-50 kDa,36 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-FoxM1 antibody [EPR30945-524] (<a href='/products/primary-antibodies/foxm1-antibody-epr30945-524-ab327650'>ab327650</a>) at 1/1000 dilution

Lane 1:

Untreated U-2 OS (human bone osteosarcoma epithelial cell) fresh whole cell lysate at 20 µg

Lane 2:

U-2 OS treated with 2μM Aphidicolin for 24h, fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 70s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the higher MW band at approximately 300 kDa is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-BCL9 antibody [EPR29651-74] (<a href='/products/primary-antibodies/bcl9-antibody-epr29651-74-ab325937'>ab325937</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control fresh whole cell lysate at 20 µg

Lane 2:

293T transfected with siRNA specifically targeting BCL9 fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 2-4 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 and lane 1 is incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

Exposure time : Lane 1 : 59 seconds; Lane 2 : 10 seconds; Lane 3 : 125 seconds; Lane 4 : 10 seconds.

All lanes:

Western blot - Anti-GSDMD antibody [EPR23078-13R] (<a href='/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

HCC1937 (human mammary gland epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 2 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 52 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-UBP43/USP18 antibody [EPR26174-540] (<a href='/products/primary-antibodies/ubp43-usp18-antibody-epr26174-540-ab325178'>ab325178</a>) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 50 µg

Lane 2:

THP-1 treated with 1 ug/ml LPS for 8 hours whole cell lysate at 50 µg

Lane 3:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

HeLa treated with 10ng/ml IFN alpha (human) for 16 hours whole cell lysate at 50 µg

Lane 5:

Untreated MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 6:

MCF7 treated with 100U/ml IFN alpha (human) for 24 hours whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 35 kDa,37 kDa,124 kDa

false

Exposure time: 6s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab327707 was shown to bind specifically to WRN. Target of interest was observed at 200 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in WRN knockout cell lysates (lane 2).

Low expression : HT-29, PANC-1, SW1116.

The identity of the bands lower than 180 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR29645-145] (<a href='/products/primary-antibodies/werners-syndrome-helicase-wrn-antibody-epr29645-145-ab327707'>ab327707</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

WRN knockout HAP1 cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

SK-N-MC (human brain epithelial cell) whole cell lysate at 20 µg

Lane 5:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

SW1116 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 8:

Ramos (human burkitt's lymphoma b lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab325178 was shown to bind specifically to UBP43/USP18. Target of interest was observed at 35 kDa and 37kDa in wide-type A549 cell lysates (lane 2) with no signal observed at this size in USP18 knockout cell line (lane 3, 4) (lane 3, knockout cell line ab267178 / knockout cell lysate ab258752).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-UBP43/USP18 antibody [EPR26174-540] (<a href='/products/primary-antibodies/ubp43-usp18-antibody-epr26174-540-ab325178'>ab325178</a>) at 1/1000 dilution

Lane 1:

Untreated wide-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

Wide-type A549 treated with 100U/ml IFN alpha (human) for 14 hours whole cell lysate at 50 µg

Lane 3:

Untreated USP18 knockout A549 whole cell lysate at 50 µg

Lane 4:

USP18 knockout A549 treated with 100U/ml IFN alpha (human) for 14 hours whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 35 kDa,37 kDa,124 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab324875 was shown to bind specifically to Tet2 . Target of interest was observed at 260 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in Tet2 knockout cell line (lane 2).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The bands beneath the target band (260 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Tet2 antibody [EPR29630-29] (<a href='/products/primary-antibodies/tet2-antibody-epr29630-29-ab324875'>ab324875</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

Tet2 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 260 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The bands beneath the target band (260 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Tet2 antibody [EPR29630-29] (<a href='/products/primary-antibodies/tet2-antibody-epr29630-29-ab324875'>ab324875</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 10 µg

Lane 2:

293T transfected with siRNA specifically targeting Tet2 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 260 kDa,124 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] ab129002 staining at 1/10000 dilution.

All lanes:

Western blot - Anti-KDM6B / JMJD3 antibody [EPR28325-184] (<a href='/products/primary-antibodies/kdm6b-jmjd3-antibody-epr28325-184-ab324740'>ab324740</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control whole cell fresh lysate at 20 µg

Lane 2:

K-562 transfected with siRNA specifically targeting KDM6B whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 200 kDa,124 kDa

true

Exposure time: 92s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KDM6B / JMJD3 antibody [EPR28325-184] (<a href='/products/primary-antibodies/kdm6b-jmjd3-antibody-epr28325-184-ab324740'>ab324740</a>) at 1/1000 dilution

Lane 1:

Untreated SW620 (human colorectal adenocarcinoma epithelial cell) whole cell fresh lysate at 20 µg

Lane 2:

SW620 treated with 80 nM TPA for 8 hours whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 200 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low/negative samples : SW480 (PMID : 31324782), HeLa (PMID : 1463733)

All lanes:

Western blot - Anti-Prothrombin antibody [RM1252] (<a href='/products/primary-antibodies/prothrombin-antibody-rm1252-ab322113'>ab322113</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 72 kDa

false

Exposure time: 20s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Exposure time : Lane 1 : 125 seconds; Lanes 2,3 and 4 : 3 minutes; Lane 5 : 70 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

Lower weak bands below 50 kDa represent other ETS1 isoforms.

All lanes:

Western blot - Anti-ETS1 antibody [EPR21909] (<a href='/products/primary-antibodies/ets1-antibody-epr21909-ab220361'>ab220361</a>) at 1/1000 dilution

Lane 1:

KARPAS-299 (human anaplastic large cell lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

Rat spleen lysate at 20 µg

Lane 4:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 5:

Mouse lymph node lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : U-2 OS, 293T, NIH/3T3

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Stathmin-2/STMN2 antibody [EPR31028-544] (<a href='/products/primary-antibodies/stathmin-2-stmn2-antibody-epr31028-544-ab326937'>ab326937</a>) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 136s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : kidney, spleen, liver, testis

Lanes 1-3 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-9 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Stathmin-2/STMN2 antibody [EPR31028-544] (<a href='/products/primary-antibodies/stathmin-2-stmn2-antibody-epr31028-544-ab326937'>ab326937</a>) at 1/1000 dilution

Lane 1:

Human cerebral cortex tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Lane 7:

Rat brain tissue lysate at 20 µg

Lane 8:

Rat liver tissue lysate at 20 µg

Lane 9:

Rat testis tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 4 - 9:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

Exposure time : Lanes 1-3 : 10 seconds, lanes 4-5 : 180 seconds

All lanes:

Western blot - Anti-INO80 antibody [EPR30384-761] (<a href='/products/primary-antibodies/ino80-antibody-epr30384-761-ab325542'>ab325542</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

CT26.WT (mouse colon fibroblast) whole cell lysate at 50 µg

Lane 5:

B16-F10 (mouse skin melanoma cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 200 kDa,124 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-RPL26+RPL26L antibody [EPR28600-70] (<a href='/products/primary-antibodies/rpl26rpl26l-antibody-epr28600-70-ab325434'>ab325434</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

RBL-2H3 (rat Peripheral blood basophil) whole cell lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Mouse thymus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 17 kDa,36 kDa

false

Exposure time: 70s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

293T cells express endogenously BCL9. This antibody does not cross-react with human BCL9L by WB.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(200KDa).

All lanes:

Western blot - Anti-BCL9 antibody [EPR29651-74] (<a href='/products/primary-antibodies/bcl9-antibody-epr29651-74-ab325937'>ab325937</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a his-tag, fresh whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human BCL9 expression vector containing a his-tag, fresh whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human BCL9L expression vector containing a his-tag, fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : MCF7, A549.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LIGHT/TNFSF14 antibody [EPR29031-53] (<a href='/products/primary-antibodies/light-tnfsf14-antibody-epr29031-53-ab323382'>ab323382</a>) at 1/1000 dilution

Lane 1:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 80 µg

Lane 2:

No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) whole cell lysate at 80 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 18-28 kDa,124 kDa

false

Exposure time: 70s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

In Western blot, Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) 1 : 1000 dilution.

All lanes:

Western blot - Anti-beta I Tubulin antibody [EPR16778] (<a href='/products/primary-antibodies/beta-i-tubulin-antibody-epr16778-ab179511'>ab179511</a>) at 1/5000 dilution

Lane 1:

HEK293T (human embryonic kidney epithelial cell) cells transfected with an empty vector whole cell lysate at 20 µg

Lane 2:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 1 whole cell lysate at 20 µg

Lane 3:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 2A whole cell lysate at 20 µg

Lane 4:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 2B whole cell lysate at 20 µg

Lane 5:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 3 whole cell lysate at 20 µg

Lane 6:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 4A whole cell lysate at 20 µg

Lane 7:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 4B whole cell lysate at 20 µg

Lane 8:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 5 whole cell lysate at 20 µg

Lane 9:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 6 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HEL.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-BCL9 antibody [EPR29651-74] (<a href='/products/primary-antibodies/bcl9-antibody-epr29651-74-ab325937'>ab325937</a>) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) fresh whole cell lysate at 20 µg

Lane 2:

HEL (human erythroleukemia erythroblast) fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed is consistent with that described in the literature (PMID : 16125763 and 30460894) Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-MAVS antibody [EPR26357-91] (<a href='/products/primary-antibodies/mavs-antibody-epr26357-91-ab290729'>ab290729</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell fresh lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 75 kDa,52 kDa,37 kDa,30 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HepG2 (PMID : 30790340).

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-ETS1 antibody [EPR21909] (<a href='/products/primary-antibodies/ets1-antibody-epr21909-ab220361'>ab220361</a>) at 1/1000 dilution

Lane 1:

KARPAS-299 (human anaplastic large cell lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 51 kDa

false

Exposure time: 180s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

This data was developed using ab133251, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-c-Rel antibody [EPR2559(2)] (<a href='/products/primary-antibodies/c-rel-antibody-epr25592-ab133251'>ab133251</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

The rat cerebellum lysate in lane 3 is prepared by 1%SDS hot lysis method. This antibody can work in RIPA and 1% SDS hot lysates in WB.

For Lysate preparation protocol, please refer to the protocol book in the protocol section.

All lanes:

Western blot - Anti-Parvalbumin antibody [EPR13091] (<a href='/products/primary-antibodies/parvalbumin-antibody-epr13091-ab181086'>ab181086</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum lysate prepared using RIPA lysis method at 20 µg

Lane 2:

Mouse cerebellum lysate prepared using 1% SDS hot lysis method at 20 µg

Lane 3:

Rat cerebellum lysate prepared using 1% SDS hot lysis method at 20 µg

Lane 4:

Human brain lysate prepared using RIPA lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

All lanes:

Western blot - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (<a href='/products/primary-antibodies/hexokinase-1-antibody-epr10134b-mitochondrial-outer-membrane-marker-ab150423'>ab150423</a>) at 1/1000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 5:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 102 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

In Western blot, Anti-DDDDK tag (Binds to FLAG^® tag sequence) antibody [EPR20018-251] (ab205606) 1/1000 dilution.

All lanes:

Western blot - Anti-beta IV Tubulin antibody [EPR16776] (<a href='/products/primary-antibodies/beta-iv-tubulin-antibody-epr16776-ab179509'>ab179509</a>) at 1/5000 dilution

Lane 1:

HEK293T (human embryonic kidney epithelial cell) cells transfected with an empty vector whole cell lysate at 20 µg

Lane 2:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 1 whole cell lysate at 20 µg

Lane 3:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 2A whole cell lysate at 20 µg

Lane 4:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 2B whole cell lysate at 20 µg

Lane 5:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 3 whole cell lysate at 20 µg

Lane 6:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 4A whole cell lysate at 20 µg

Lane 7:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 4B whole cell lysate at 20 µg

Lane 8:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 5 whole cell lysate at 20 µg

Lane 9:

HEK293T (human embryonic kidney epithelial cell) + OE- Tubulin beta- 6 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human DLL3 or DLL4 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(85, 30KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-DLL1 antibody [EPR29655-26] (<a href='/products/primary-antibodies/dll1-antibody-epr29655-26-ab327088'>ab327088</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human DLL1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human DLL3 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human DLL4 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 85 kDa,30 kDa,36 kDa

false

Exposure time: 8s