AB96886

Goat Anti-Rabbit IgG H&L (DyLight® 650)

Name: Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886) | Abcam
Brand: Abcam Limited
SKU: AB96886
Price: 41000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(10 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Cited in 10 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

19 Images

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HT-29 cells permeabilized with 90% methanol labeling human SMC1 with ab243875 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Detection of human E-Cadherin (shaded) in HT29 cells by flow cytometry. Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] or isotype control (unshaded). Secondary : DyLight^® 650-conjugated goat anti-rabbit IgG (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling mouse beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed Daudi cells permeabilized with 90% methanol labeling human TFEB with ab264421 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human MED1 with ab243893 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling human beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed K562 cells permeabilized with 90% methanol labeling human CTCF with ab243897 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HEK293T cells permeabilized with 90% methanol labeling mouse B7-H3/CD276 with ab243882 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed K562 cells permeabilized with 90% methanol labeling human BRD3 with ab264420 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Detection of human Granzyme B (shaded) in SR (human lymphoma cell line) cells by flow cytometry.

SR cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10⁶ cells.

Antibody : Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (ab243879) or isotype control (unshaded).

Secondary : DyLight® 650-conjugated goat anti-rabbit IgG (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed mIMCD-3 cells permeabilized with 90% methanol labeling human 53BP1 with ab243868 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details, Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed NIH3T3 cells permeabilized with 90% methanol labeling mouse B7-H3/CD276 with ab243882 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed A20 cells permeabilized with 90% methanol labeling mouse CTCF with ab243897 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed NIH3T3 cells permeabilized with 90% methanol labeling human SMC1 with ab243875 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed mIMCD-3 cells permeabilized with 90% methanol labeling human MED1 with ab243893 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Key facts

宿主種

Goat

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

血清吸着処理済み

標識

DyLight® 650

励起波長/蛍光波長

Ex: 654nm, Em: 673nm

アプリケーション

IHC-P, Flow Cyt, ICC/IF

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments." } } }

style

left-column

製品の詳細

DyLight® 650 replaces DyLight® 649.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

style

left-column

style

left-column

style

left-column

製品プロトコール

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

See full target information Rabbit IgG

style

left-column

文献 (10)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0316443 PubMed40029831

2025

Understanding the early molecular changes associated with radiation therapy-A preliminary bulk RNA sequencing study.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Miller,Henning De May,David L Rou,Jayant P Agarwal,Sujee Jeyapalina

Blood cancer journal 13:125 PubMed37591861

2023

The oncogenic human B-cell lymphoma MYD88 L265P mutation genocopies activation by phosphorylation at the Toll/interleukin-1 receptor (TIR) domain.

Applications

Unspecified application

Species

Unspecified reactive species

Marthe Minderman,Hildo Lantermans,Carmen van der Zwaan,Arie J Hoogendijk,Maartje van den Biggelaar,Marie José Kersten,Marcel Spaargaren,Steven T Pals

BMC molecular and cell biology 23:26 PubMed35794526

2022

Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Wei Lee,Chia-Chen Kuo,Chi-Jung Liang,Huei-Jyuan Pan,Chia-Ning Shen,Chau-Hwang Lee

British journal of pharmacology 179:1753-1768 PubMed34825370

2022

Sphingosine-1-phosphate/TGF-β axis drives epithelial mesenchymal transition in asthma-like disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Riemma,Ida Cerqua,Barbara Romano,Elena Irollo,Antonio Bertolino,Rosa Camerlingo,Elisabetta Granato,Giuseppina Rea,Stefania Scala,Michela Terlizzi,Giuseppe Spaziano,Rosalinda Sorrentino,Bruno D'Agostino,Fiorentina Roviezzo,Giuseppe Cirino

iScience 24:103018 PubMed34522861

2021

Encephalopathy-causing mutations in Gβ () alter regulation of neuronal GIRK channels.

Applications

Unspecified application

Species

Unspecified reactive species

Haritha P Reddy,Daniel Yakubovich,Tal Keren-Raifman,Galit Tabak,Vladimir A Tsemakhovich,Maria H Pedersen,Boris Shalomov,Sophie Colombo,David B Goldstein,Jonathan A Javitch,Amal K Bera,Nathan Dascal

International journal of molecular sciences 20: PubMed31717998

2019

Mechanisms of Neuronal Death in the Cerebral Cortex during Aging and Development of Alzheimer's Disease-Like Pathology in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Darya V Telegina,Gleb K Suvorov,Oyuna S Kozhevnikova,Nataliya G Kolosova

Scientific reports 9:6749 PubMed31043642

2019

G protein-coupled estrogen receptor mediates anti-inflammatory action in Crohn's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Damian Jacenik,Marta Zielińska,Anna Mokrowiecka,Sylwia Michlewska,Ewa Małecka-Panas,Radzisław Kordek,Jakub Fichna,Wanda M Krajewska

Scientific reports 9:508 PubMed30679535

2019

Mutual action by Gγ and Gβ for optimal activation of GIRK channels in a channel subunit-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Galit Tabak,Tal Keren-Raifman,Uri Kahanovitch,Nathan Dascal

Aging 8:2713-2733 PubMed27750209

2016

An antioxidant specifically targeting mitochondria delays progression of Alzheimer's disease-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia A Stefanova,Natalia A Muraleva,Kseniya Yi Maksimova,Ekaterina A Rudnitskaya,Elena Kiseleva,Darya V Telegina,Nataliya G Kolosova

Journal of pineal research 59:163-77 PubMed25988948

2015

Melatonin attenuates impairments of structural hippocampal neuroplasticity in OXYS rats during active progression of Alzheimer's disease-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia A Stefanova,Kseniya Y Maksimova,Elena Kiseleva,Ekaterina A Rudnitskaya,Natalia A Muraleva,Nataliya G Kolosova

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (DyLight® 650)