AB96899

Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

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(139 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 139 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

4 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96899)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96899)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX (ab120017), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120017 (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight^® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96899)

Effects of FZE on apoptotic ratio and apoptotic factors in RSC96 cells.

Effects of FZE on translocation of CytoC and the levels of caspase9 and caspase3. The cells were fixed with 4% paraformaldehyde for 15 minutes at 20°C, permeated with 0.3% triton prior to being blocked in 1% BSA+2% normal goat serum for 30 min at 20°C. Samples were then incubated with primary antibody overnight at 4°C in PBS containing. ab96899 diluted at 1∶200 was used as the secondary antibody. Cell nucleus were counterstained with DAPI and showed blue. Mitochondria were labeled by Mito tracker and showed red.

Han et al PLoS One. 2014 Jan 23;9(1):e86539. doi: 10.1371/journal.pone.0086539. eCollection 2014. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96899)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96899)

Overlay histogram showing Raji cells stained with ab52922 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52922, 1/100) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96899)

All lanes:

Cocktail of rabbit anti-Actin and mouse anti-hnRNP at 1 µg/mL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899) at 0.5 µg/mL

false

Exposure time: 42s

Key facts

宿主種

Goat

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

Mouse, Rat, Goat, Horse, Chicken, Cow, Human, Pig

血清吸着処理済み

Yes

標識

DyLight® 488

励起波長/蛍光波長

Ex: 493nm, Em: 518nm

アプリケーション

Flow Cyt, IHC-P, ICC/IF, WB

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/20000", "notes":"5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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文献 (139)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:2509 PubMed38291199

2024

Oral administration of ellagic acid mitigates perioperative neurocognitive disorders, hippocampal oxidative stress, and neuroinflammation in aged mice by restoring IGF-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Chen,Kai Lu,Ning Bai,Yabo Hao,Hui Wang,Xinrong Zhao,Fang Yue

Frontiers in cell and developmental biology 11:1308879 PubMed38269087

2024

Glycocalyx analysis of bladder cancer: three-dimensional images in electron microscopy and vicia villosa lectin as a marker for invasiveness in frozen sections.

Applications

Unspecified application

Species

Unspecified reactive species

Torai Enomoto,Hideshi Okada,Hiroyuki Tomita,Koji Iinuma,Keita Nakane,Yuki Tobisawa,Akira Hara,Takuya Koie

Oncology research 32:213-226 PubMed38188679

2024

Silencing of peroxiredoxin 2 suppresses proliferation and Wnt/β-catenin pathway, and induces senescence in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xuegang Yang,Xianhong Xiang,Guohui Xu,Shi Zhou,Tianzhi An,Zhi Huang

Nature communications 14:8217 PubMed38086852

2023

Nuclear cGAS restricts L1 retrotransposition by promoting TRIM41-mediated ORF2p ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyi Zhen,Yu Chen,Haiyan Wang,Huanyin Tang,Haiping Zhang,Haipeng Liu,Ying Jiang,Zhiyong Mao

Cells 12: PubMed37947632

2023

Myocardial Mitochondrial DNA Drives Macrophage Inflammatory Response through STING Signaling in Coxsackievirus B3-Induced Viral Myocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Andong Qin,Zhenke Wen,Sidong Xiong

International journal of experimental pathology 105:4-12 PubMed37899670

2023

Notoginsenoside R1 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via ERα/GSK-3β/β-catenin signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lu,Yuanxin Shi,Minglei Qian

Experimental and therapeutic medicine 26:531 PubMed37869648

2023

FRZB affects aureus‑induced osteomyelitis in human bone marrow derived stem cells by regulating the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Wenyong Pang,Hongsong Fan,Hao Wang,Leibing Zhang

Frontiers in immunology 14:1258344 PubMed37744335

2023

Multi-omics analysis reveals the association between elevated KIF18B expression and unfavorable prognosis, immune evasion, and regulatory T cell activation in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Tang,Zhenyu Wu,Lusi Chen,Longjiang She,Weihan Zuo,Weijun Luo,Yang Zhang,Shaoqiang Liang,Guichao Liu,Biyi He,Jinfeng He,Ning Zhang

Cellular and molecular biology (Noisy-le-Grand, France) 69:198-202 PubMed37715384

2023

Nuclear factor-kappa B p50-induced microRNA-20a-3p plays a detrimental role in sepsis-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Weihong Mao,Xiaoming Wang,Yongchen Zhang,Huding Zhu,Li Dai,Juanjuan Chen

Analytical cellular pathology (Amsterdam) 2023:5852963 PubMed37234237

2023

LINC00659 Inhibits Hepatocellular Carcinoma Malignant Progression by Blocking Aerobic Glycolysis through FUS Recruitment and SLC10A1 Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Chen,Xin Xu,Wei Wu,Ke Zheng,Yijun Yu

View all publications

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Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed