AB6940

Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

Name: Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) | Abcam
Brand: Abcam Limited
SKU: AB6940
Price: 42000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(47 Publications)

Goat Anti-Rabbit IgG H&L (Cy2 ®) preabsorbed (ab6940) is designed to minimize non-specific binding and high background staining. Suitable for Western blot Flow Cytometry, IHC, ICC/IF and ELISA applications.

- Proven performance: cited in over 45 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images

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Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

ab6940 was used at dilution 1/10000 with the primary antibody ab47840 in WB. See the review on ab47840.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/10000 dilution

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Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with the primary antibody for 1 hour at room temperature.

Incubated with the secondary antibody for 30 minutes at room temperature.

Lane 1:

Rabbit IgG whole molecule at 0.05 µg

Lane 2:

Rabbit IgG F(ab) fragment at 0.05 µg

Lane 3:

Rabbit IgG F(c) fragment at 0.05 µg

Lane 4:

Rabbit IgM whole molecule at 0.05 µg

Lane 5:

Normal rabbit serum at 0.05 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

Lanes 1 - 5:

Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution

false

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with ab6940 for 1 hour at room temperature.

All lanes:

Rabbit IgG at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

false

Key facts

宿主種

Goat

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

Mouse, Rat, Sheep, Goat, Horse, Guinea pig, Hamster, Cow, Human, Chicken

血清吸着処理済み

Yes

標識

Cy2®

励起波長/蛍光波長

Ex: 489nm, Em: 505nm

アプリケーション

ICC, IHC-P, Flow Cyt, IHC-Fr, ELISA, WB, ICC/IF

applications

クローン性

Polyclonal

アイソタイプ

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" } } }

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製品の詳細

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.

バッファー組成

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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文献 (47)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 16:20417314251326668 PubMed40342587

2025

Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Mercedes Olvera-Valencia,Verónica Garcia-Castillo,Rosalío Ramos-Payan,Maribel Aguilar-Medina,Samuel Trujano-Camacho,Alejandro López-Saavedra,Laurence A Marchat,Cesar López-Camarillo,Ronen Sumagin,Eloy Pérez-Yepez,Carlos Pérez-Plasencia

Communications biology 8:514 PubMed40155473

2025

Defining gastric cancer ecology: the crucial roles of TREM2 macrophages and fibroblasts in tumor microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Cao,Dianshui Sun,Can Tu,Jihua Wang,Runjia Fu,Rumei Gong,Yueying Xiao,Qin Liu,Xiaomei Li

Molecular biology reports 52:302 PubMed40080230

2025

Simultaneous isolation and culture of endothelial colony-forming cells, endothelial cells and vascular smooth muscle cells from human umbilical cords.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Lotus Burger,Steeve Menétrey,Catherine Ponti,Karine Lepigeon,Joanna Sichitiu,Anne-Christine Peyter

Bio-protocol 15:e5181 PubMed39959295

2025

Development and Validation of Mouse Models for Studying Genital Tract Infection Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Wang,Zixuan Han,Luying Wang,Xin Sun,Qi Tian,Tianyuan Zhang

World journal of gastroenterology 30:5174-5190 PubMed39735272

2024

N6-methyladenosine-modified long non-coding RNA promotes stemness and sorafenib resistance in hepatocellular carcinoma by upregulating SHOX2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yu,Xiang-Hong Lu,Jin-Song Mu,Jiang-Yun Meng,Jiang-Shan Sun,Hai-Xu Chen,Yang Yan,Ke Meng

Cells 13: PubMed39404412

2024

Laherradurin Inhibits Colorectal Cancer Cell Growth by Induction of Mitochondrial Dysfunction and Autophagy Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Izamary Delgado-Waldo,Svetlana Dokudovskaya,Yahir A Loissell-Baltazar,Eduardo Pérez-Arteaga,Jossimar Coronel-Hernández,Mariano Martínez-Vázquez,Eloy Andrés Pérez-Yépez,Alejandro Lopez-Saavedra,Nadia Jacobo-Herrera,Carlos Pérez Plasencia

The International journal of developmental biology 68:19-24 PubMed38591690

2024

Disrupted odontoblast differentiation and dentin dysplasia in -deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Jiménez-Rojo,Susana de Vega,Gaskon Ibarretxe,Takashi Nakamura,Fernando J Unda

Drug metabolism and disposition: the biological fate of chemicals 51:1230-1237 PubMed37349114

2023

NEAT1_2 and DAZAP1, Paraspeckle Components, Interact with PXR to Negatively Regulate CYP3A4 Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Rei Mitamura,Masataka Nakano,Motoki Isono,Kiamu Kurosawa,Tatsuki Fukami,Miki Nakajima

Theranostics 13:3550-3567 PubMed37441598

2023

Label-free optical metabolic imaging of adipose tissues for prediabetes diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Chen,Guihui Qin,Yuhong Liu,Moxin Li,Yue Li,Lun-Zhang Guo,Lidong Du,Weiming Zheng,Pei-Chun Wu,Yueh-Hsun Chuang,Xiaoyan Wang,Tzung-Dau Wang,Ja-An Annie Ho,Tzu-Ming Liu

Frontiers in microbiology 14:1142283 PubMed37125189

2023

Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tian,Tianyuan Zhang,Luying Wang,Jingyue Ma,Xin Sun

View all publications

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Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed