Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

ICC/IF image of beta Tubulin staining in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab150077 Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling Sall4 with ab315176 at 1/100 (5.07 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing cytoplasmic staining in NCCIT cell line. Negative control : 293T. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1ug/ ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/mL) dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CSNK2A1 with purified ab76040 at 1 : 20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PKC beta 2 using ab32026. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab32026 at 1 : 50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (shown in red). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling Histone H3 (acetyl K27) with ab177178 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing increased nuclear staining in HeLa cells treated with TSA (500 ng/ml, 4 hours).

The nuclear counter stain is DAPI (blue).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left panel) / Caco-2 (Human colorectal adenocarcinoma epithelial cell, Right panel) using ab278053 at 1/500 dilution (0.1µg) (red). The isotype control used was the Rabbit monoclonal IgG (ab172730), black line and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Secondary antibody used was the Goat anti-rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution.

Negative control : HEK-293T. (PMID 20167130)
Gated on viable cells.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/ Immunofluorescence analysis of PBMC (Human primary peripheral blood mononuclear cell) cells labeling IL-2 using ab92381. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab92381 at 1 : 50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (shown in red). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemical/immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma- astrocytoma epithelial cell) cells labelling AKR1C1/AKR1C2 with primary antibody anti-AKR1C1/AKR1C2 (ab179448) at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in U-87 MG cell line. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1 : 200 dilution. The nuclear counter stain is DAPI (blue).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling LATS1/WARTS with ab243656 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling IMP3 using ab177477. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab177477 at 1 : 50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (shown in red). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) labeling BMAL1 with ab230822 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) (ab150077) secondary antibody at 1/1000 dilution (green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain.

The nuclear counterstain is DAPI (blue).

Confocal image showing positive staining in PC-3 cell line.

Negative Control : Raji (PMID : 29230015).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized Wildtype HEK-293T (human embryonic kidney epithelial cell), and RAB13 KO HEK293T (RAB13 knockout HEK293T) cells labelling RAB13 with ab205528 at 1/100 (11.06 μg/ml) followed by ab150077 Goat Anti-Rabbit (Alexa Fluor^® 488) as secondary at 1/1000 (2 μg/ml) dilution. Confocal image showing cytoplasmic and membranous staining in wildtype HEK-293T (shown in green), showing no staining in RAB13 knockout HEK-293T cells. ab7291 Anti-alpha Tubulin antibody [DM1A] - Loading Control was used as a counterstain at 1/200 with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed as secondary (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling HLA A with purified ab52922 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) labeling CD239/BCAM with ab134110 at 1/100 dilution followed by ab150077 at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilied with 0.1% TritonX-100. DAPI was used as nuclear counter stain. Cells were counterstained wth ab195889 at 1/200 dilution. Confocal image showing mainly membranous staining in A431 cell line. Negative control : JurKat (PMID : 30078440) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized U-87 MG cells labelling HOXB5 with ab284782 at 1/500 dilution , followed by Alexa Fluor^® 488-conjugated Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (Green).

Cells were incubated overnight at 4° C, followed by Alexa Fluor^® 488-conjugated Goat anti-Rabbit secondary antibody (ab150077) at RT for 45 min. ab7291 Anti-beta Tubulin, used as a counterstain at 1/1000 dilution, was co-incubated with ab284782 overnight at 4° C, followed by Alexa Fluor^® 594 Goat Anti-Mouse secondary (ab150120) at 1/1000 dilution at RT for 45 min (Magenta). Nucleus were visualized using DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized Wildtype HEK-293T (human embryonic kidney epithelial cell), and RAB13 KO HEK293T (RAB13 knockout HEK293T) cells labelling RAB13 with ab180936 at 1/100 (10.75 μg/ml) followed by ab150077 Goat Anti-Rabbit (Alexa Fluor^® 488) as secondary at 1/1000 (2 μg/ml) dilution. Confocal image showing cytoplasmic and membranous staining in wildtype HEK-293T (shown in green), showing no staining in RAB13 knockout HEK-293T cells. ab7291 Anti-alpha Tubulin antibody [DM1A] - Loading Control was used as a counterstain at 1/200 with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed as secondary (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) labelling ROR beta/RORB with ab187657 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488 (ab150077) secondary antibody at 1/1000 dilution (green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution was used as a counterstain (red).

The nuclear counterstain is DAPI (blue).

Confocal image showing nuclear staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CLDN1 KO A431(CLDN1 knockout human epidermoid carcinoma epithelial cell) cells labelling Claudin 1 with ab307692 at 1/250 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution (Green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) ( ab195889 ) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is AlexaFluor488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Confocal image showing membranous and cytoplasmic staining in wildtype A431 cells and showing no staining in CLDN1 knockout A431 cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Intracellular Flow Cytometry analysis of 293T (human embryonic kidney epithelial cell) transfected with myc-tagged PADI4 construct, then treated with 10mM CaCl2 and 10uM Ionomycin for 4h labeling Histone H3 (citrulline R2 + R8 + R17) with ab281584 at 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control : Rabbit monoclonal IgG (ab172730)/left.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry analysis of Raji (human Burkitt's lymphoma B lymphocyte) labeling PLCG 2 with purified ab51020 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.12 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labeling alpha smooth muscle Actin (green) with purified ab32575 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 Alexa Fluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) (+ve cell line), and HL-60 (human acute promyelocytic leukemia promyeloblast) (Low expressing cell line) labelling RAB13 with ab180936 at 1/100 (10.75 μg/ml) followed by ab150077 Goat Anti-Rabbit (Alexa Fluor^® 488) as secondary at 1/1000 (2 μg/ml) dilution. Confocal image showing mainly cytoplasmic staining in U-87 MG cell line (shown in green). ab7291 Anti-alpha Tubulin antibody [DM1A] - Loading Control was used as a counterstain at 1/200 with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed as secondary (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). Low expression : HL-60. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) (+ve cell line), and HL-60 (human acute promyelocytic leukemia promyeloblast) (Low expressing cell line) labelling RAB13 with ab205528 at 1/100 (11.06 μg/ml) followed by ab150077 Goat Anti-Rabbit (Alexa Fluor^® 488) as secondary at 1/1000 (2 μg/ml) dilution. Confocal image showing mainly cytoplasmic staining in U-87 MG cell line (shown in green). ab7291 Anti-alpha Tubulin antibody [DM1A] - Loading Control was used as a counterstain at 1/200 with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed as secondary (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). Low expression : HL-60. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 5 μg/ml (1/200 dilution). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an AlexaFluor^®488 Goat anti-Rabbit was used as the secondary antibody at 2 μg/ml (1/1000 dilution). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain at 2.5 μg/ml (1/200 dilution). DAPI nuclear counterstain.
Confocal image showing the signal increased after EGF (100ng/ml, 5 min) treatment and decreased after Lambda Protein Phosphatase treatment (31°C for 2 hours).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling KDM3B / JMJD1B with ab271046 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubμue Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast adenocarcinoma epithelial cell) labeling 15-PGDH with ab187160 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain (red).

The nuclear counterstain is DAPI (blue).

Confocal image showing mainly cytoplasmic staining in MCF-7 cell line.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized LAMP1 KO HAP1 cells labelling LAMP1 with ab278043 at 1/100 (5.45 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (green). Confocal image showing cytoplasmic staining in parental HAP1 cell line and no staining in LAMP1 KO HAP1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody- Microtubule Marker (Alexa Fluor ^® 594) was used to counterstain at 1/200 (red).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

ab64085 staining TMEM16A in PC-3 (human prostate adenocarcinoma epithelial cell) cells. The cells were fixed with 4% formaldehyde, permeabilized in 100% methanol. The cells were then incubated with ab64085 at 1/20 dilution, followed by secondary antibody ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used for counterstain at 1/200 dilution (Red). Nuclear DNA was labelled in blue with DAPI. Confocal image showing membranous and cytoplasmic staining in PC-3 cell line. Low expression control : LnCaP(PMID : 29899325) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling LAMP1 with ab278043 at 1/100 (5.45 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (green). Confocal image showing cytoplasmic staining in HeLa. ab25630 Anti-LAMP1 mouse monoclonal antibody was used to counterstain at 1/500 (red). The Nuclear counterstain was DAPI (blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged IL-21 construct cells labelling Il21 with ab227837 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100 HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SERPINB1/PI2 with ab190357 at 1/100 dilution (10 μg/ml), followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (2 μg/ml) (green). Confocal image showing cytoplasmic staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). DAPI was used as nuclear counterstain. The negative controls are as follows : -ve control 1 : ab190357 at 1/100 dilution (10 μg/ml), followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (2 μg/ml). -ve control 2 : AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (2 μg/ml).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CTNNB1 KO HAP1 (CTNNB1 knockout human chronic myelogenous leukemia near-haploid cell line) cells labelling beta Catenin non-phospho S37/T44 with ab246504 at 1/100 (5.4 μg/ml) dilution, followed by ab150077 antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing membranous staining in parental HAP1 cell line, and staining in the CTNNB1 KO HAP1 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150077 at 1/1000 (2 μg/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Ramos cells with or without treatment labelling CD22 (phospho Y822) with ab32123 at 1/200 dilution followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (Green). Confocal images showing no staining in Ramos cells and strong membranous staining in Ramos treated with pervanadate (1mM 30min) but weak membranous staining in Ramos treated with pervanadate (1mM 30min) and phosphatase.
ab7291 Anti-beta Tubulin, used as a counterstain at 1/1000 dilution, was co-incubated with ab32123 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Mouse secondary (ab150120) at 1/1000 dilution at RT for 45 min (Magenta). Nucleus were visualized using DAPI.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 25 ug/mL anisomycin for 30 min, then Lambda Protein Phosphatase 31 for 2 hours cells labeling Hsp27 with purified ab32501 at 1/50 dilution (11.3 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) dilution. Goat anti-rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATG9A with Purified ab108338 at 1/100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling RNF20 with ab181104 at 1/200 (8.5 μg/ml) dilution, followed by ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) labeling FABP5 with ab255276 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain (red).

The nuclear counterstain is DAPI (blue).

Confocal image showing cytoplasmic and nuclear staining in MDA-MB-231 cells.

Negative Control : T-47D (PMID : 21356353).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma) cells labelling CD63 with ab315108 at 1/100 (5.24 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in SK-MEL-28 cell line. Low expression cell line : Jurkat. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry analysis of Jurkat (human T cell leukemia T lymphocyte) labeling p95/NBS1 with purified ab109453 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.27 μg/ml) was used as counterstain. Nuclei were stained blue with DAPI. Negative control : PBS instead of the primary antibody.

Confocal image showing increased nuclear staining in Jurkat cells treated with Etoposide (25uM) for 2 h.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Intracellular Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PSMA with ab247436 at 1/100 dilution (1 µg) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling NDRG1 using ab124689. The cells were fixed with 100% Methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab124689 at 1 : 50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (shown in red). Secondary antibody only control : PBS instead of the primary antibody.