AB150097

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed

Name: Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097) | Abcam
Brand: Abcam Limited
SKU: AB150097
Price: 36000 JPY
Availability: InStock

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(4 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

7 Images

Lab

Immunocytochemistry - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling eIF4A3 with ab180573 at 1/90 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SD Rat primary neuron cells labelling Pan Trk with ab267830 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG Fc (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized ICR Mouse primary neuron cells labelling Pan Trk with ab267830 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG Fc (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (green) was ab150091 Alexa Fluor® 488 goat anti-rabbit IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150091 Alexa Fluor® 488 goat anti-rabbit IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Key facts

宿主種

Goat

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Fc region

最小限の交差反応性

Mouse, Rat, Human

血清吸着処理済み

Yes

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

アプリケーション

ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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製品の詳細

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Drug development research 84:1739-1750 PubMed37769152

2023

Fluoxetine enhances the antitumor effect of olfactory ensheathing cell-thymidine kinase/ganciclovir gene therapy in human glioblastoma multiforme cells through upregulation of Connexin43 levels.

Applications

Unspecified application

Species

Unspecified reactive species

Saereh Hosseindoost,Ahmad R Dehpour,Samaneh Dehghan,Seyed A H Javadi,Babak Arjmand,Ali Fallah,Mahmoudreza Hadjighassem

Experimental eye research 210:108643 PubMed34058231

2021

Luteolin inhibits the proliferation, adhesion, migration and invasion of choroidal melanoma cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Lin Shi,Yu-Fen Chen,Wei-Qi Wu,Yao Lai,Qi Jin,Wan-Lu Qiu,Dong-Lian Yu,Yi-Zhong Li,Hong-Fei Liao

Andrologia 52:e13352 PubMed31746491

2019

H4S1ph, an alternative epigenetic marker for sperm maturity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohui Zhang,Shumei Mu,Tingrong Chen,Zhe Sun,Zhiquan Shu,Yanqin Li,Xianjiang Kang

Scientific reports 6:25089 PubMed27121047

2016

Dynamics of histone H2A, H4 and HS1ph during spermatogenesis with a focus on chromatin condensation and maturity of spermatozoa.

Applications

Species

Unspecified reactive species

Zhao-Hui Zhang,Shu-Mei Mu,Ming-Shen Guo,Jiang-Li Wu,Yan-Qin Li,Han Zhang,Ying Wang,Xian-Jiang Kang

View all publications

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Abcam product promise

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Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed