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Goat Anti-Rabbit IgA alpha chain (ab97186)

Submit a review Q&A (1)References (2)

Key features and details

  • Goat Anti-Rabbit IgA alpha chain
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Conjugates logo Related conjugates and formulations

Biotin DyLight® 488 FITC HRP

製品の概要

  • 製品名

    Goat Anti-Rabbit IgA alpha chain
    IgA 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rabbit

  • 特異性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgA.

  • アプリケーション

    適用あり: ICC, IHC-P, ELISA, WBmore details

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab97186の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/100 - 1/500. for coating, 1/1000 - 1/30000 as primary
WB
1/1000 - 1/10000. colorimetric, 1/1000 - 1/30000 for Chemiluminescent detection
特記事項
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/100 - 1/500. for coating, 1/1000 - 1/30000 as primary
WB
1/1000 - 1/10000. colorimetric, 1/1000 - 1/30000 for Chemiluminescent detection

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab97186 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab97186 は 2 報の論文で使用されています。

  • Pasternak JA  et al. Intrauterine vaccination induces a dose-sensitive primary humoral response with limited evidence of recall potential. Am J Reprod Immunol 80:e12855 (2018). PubMed: 29607560
  • Liu S  et al. The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis. Oncotarget 8:66528-66539 (2017). PubMed: 29029533

レビューと Q&A

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Question


Would you be able to tell me what is the source of antigen for the anti-rabbit IgA antibodies (for instance, cat no. ab97186)?
As you may be aware, rabbits express 13 different heavy chains/isotypes as opposed to mice and humans who express 1 and 2 resp. I wanted to know which/how many isotypes this secondary antibody recognized?

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Answer

Thank you for your reply.

We do not make ab97186 in-house, so the source of of the antigen is not available.

Here are more details about the procedure: Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgA. This antibody may cross react with IgA from other species.

I hope this information helps.

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