Goat Anti-Mouse IgG1 (HRP)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) (AB97240)

IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

PubMed

Western blot - Goat Anti-Mouse IgG1 (HRP) (AB97240)

The band corresponding to the enzyme–substrate cleaved complex, has been indicated by an asterisk.

All lanes:

Anti-Topoisomerase I antibody (ab58313)

Lane 1:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 1 minute.

Lane 2:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 2 minutes.

Lane 3:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 4 minutes.

Lane 4:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 8 minutes.

Lane 5:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 1 minute.

Lane 6:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 2 minutes.

Lane 7:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 4 minutes.

Lane 8:

Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 8 minutes.

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG1 (HRP) (ab97240)

false

Image from Arnò B et al., PLOS One, Fig. 2.; doi: 10.1371/journal.pone.0068404 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/