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Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

Submit a review Q&A (2)References (55)
Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

    Key features and details

    • Goat Anti-Mouse IgG H&L (TRITC)
    • Conjugation: TRITC. Ex: 547nm, Em: 572nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, WB, Dot blot, ELISA

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer PE Texas Red ® Unconjugated Unconjugated

    製品の概要

    • 製品名

      Goat Anti-Mouse IgG H&L (TRITC)
      IgG 二次抗体 製品一覧

    • 由来種

      Goat

    • ターゲット生物種

      Mouse

    • アプリケーション

      適用あり: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, WB, Dot blot, ELISAmore details

    • 免疫原

      Mouse IgG whole molecule

    • 標識

      TRITC. Ex: 547nm, Em: 572nm

    製品の特性

    • 製品の状態

      Lyophilized:Reconstitute with 1 ml of water.

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.2
      Preservative: 0.01% Sodium azide
      Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
    • Concentration information loading...

    • 精製度

      Affinity purified

    • 特記事項（精製）

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.

    • 特記事項（標識）

      Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6786の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000 - 1/4000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000. (PubMed:15659422)
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    特記事項
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000 - 1/4000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000. (PubMed:15659422)
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.

    画像

    • Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
      Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
      Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (55)

    ab6786 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6786 は 55 報の論文で使用されています。

    • Cheng DW  et al. Emodin inhibits HDAC6 mediated NLRP3 signaling and relieves chronic inflammatory pain in mice. Exp Ther Med 27:44 (2024). PubMed: 38144917
    • Yang HY  et al. Emodin suppresses oxaliplatin-induced neuropathic pain by inhibiting COX2/NF-κB mediated spinal inflammation. J Biochem Mol Toxicol 37:e23229 (2023). PubMed: 36184831
    • Fang Z  et al. Gasdermin D affects aortic vascular smooth muscle cell pyroptosis and Ang II-induced vascular remodeling. Heliyon 9:e16619 (2023). PubMed: 37303505
    • Ji J  et al. Triple‑negative breast cancer cells that survive ionizing radiation exhibit an Axl‑dependent aggressive radioresistant phenotype. Exp Ther Med 26:448 (2023). PubMed: 37614420
    • Xu L  et al. Galectin-3 Enhances Osteogenic Differentiation of Precursor Cells From Patients With Diffuse Idiopathic Skeletal Hyperostosis via Wnt/β-Catenin Signaling. J Bone Miner Res 37:724-739 (2022). PubMed: 35064940
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    I bought the primary antibody (Primary antibody (mouse anti CK3): https://www.abcam.com/Cytokeratin-3-antibody-AE5-ab77869.html) and secondary antibody (goat anti-mouse 1)https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-FITC-pre-adsorbed-ab97039.html
    2) https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6786.html) from you.

    And I used rabbit cornea as the control and I tried several times for the positive control and negative control. But the results were weird.










    All the slides which stained with FITC were positive (for negative control also showed positive staining)

    All the slides which stained with TRITC were negative (for positive control also showed negative staining)



    And I attach the immunostaining fotos and the protocol what I used for the immunostaing in the attachment. Could you please help me check if the antibodies what I chosen are not proper or the protocol what I used is not proper?? If it is, please send me a proper protocol for the immunostaining ! Thank you very much for your help!

    Read More
    Answer

    Thank you for contacting Abcam.

    I will be happy to help you further in this matter, but I just have a few more questions for you:

    1 - Were you staining tissue sections or a monolayer of cultured cells?

    2 - If you were staining sections, how were they prepared (PfA fixation & paraffin embedded, MeOH etc)?

    3 - If these samples were paraffin embedded, what type of antigen retrieval did you use on them?

    4 - Have you used either of the secondary antibodies successfully with other primary antibodies?

    I look forward to your reply and helping to resolve these issues.

    Read More

    Question

    I would just like to know if your secondary antibody ab6786 (goat polyclonal to mouse IgG H&L conjugated to Rhodamine) would be suitable in conjunction with antibody ab8329 (EBNA 1 antibody, mouse monoclonal IgG2b). It is not listed in your list of recommended secondaries for this antibody, but I cannot see from the data sheet why it would not work, Please advise,

    Read More
    Answer

    Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

    Read More

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