Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WB
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Glucose Oxidase
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HRP
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PE
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Unconjugated
Unconjugated
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (FITC)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details -
免疫原
mouse whole molecule
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標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC (PFA fixed) |
1/1000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
1/500 - 1/2500. PubMed: 17989178
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
|
|
| ICC/IF | (1) |
1/1000 - 1/5000. (PMID16006199)
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
1/10000. PubMed: 17217624
|
| 特記事項 |
|---|
|
IHC (PFA fixed)
1/1000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
1/500 - 1/2500. PubMed: 17989178 |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365. |
|
ICC/IF
1/1000 - 1/5000. (PMID16006199) |
|
ELISA
1/10000 - 1/50000. |
|
WB
1/10000. PubMed: 17217624 |
画像
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)This image is courtesy of an Abreview submitted by J Chai.
ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
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Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785) -
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (536)
ab6785 は 536 報の論文で使用されています。
- Fan W & Zhou J Icariside II protects dopaminergic neurons from 1‑methyl‑4‑phenylpyridinium‑induced neurotoxicity by downregulating HDAC2 to restore mitochondrial function. Exp Ther Med 27:40 (2024). PubMed: 38125349
- Zhang MW et al. Curcumin relieves oxaliplatin-induced neuropathic pain via reducing inflammation and activating antioxidant response. Cell Biol Int 48:872-882 (2024). PubMed: 38480956
- Srivastav S et al. Motor neuron activity enhances the proteomic stress caused by autophagy defects in the target muscle. PLoS One 19:e0291477 (2024). PubMed: 38166124
- Huang JM et al. CX3CL1/CX3CR1 Signaling Mediated Neuroglia Activation Is Implicated in the Retinal Degeneration: A Potential Therapeutic Target to Prevent Photoreceptor Death. Invest Ophthalmol Vis Sci 65:29 (2024). PubMed: 38231527
- Le Gall-Lanotto C et al. Road-traffic-related air pollution contributes to skin barrier alteration and growth defect of sensory neurons. Exp Dermatol 33:e15009 (2024). PubMed: 38284185