Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Fr
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HRP
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PE
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Unconjugated
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製品の概要
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製品名
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Frmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Full length native protein (purified) corresponding to IgG.
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標識
Cy2 ®. Ex: 489nm, Em: 505nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
特記事項（標識）
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 5.1 moles Cy2 per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
特記事項
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6944の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
WB
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
画像
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Western blot - Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)Secondary
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944) at 1/1000 dilution (60 minutes at Room temperature)
Observed band size: 25, 55 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab6944 は 11 報の論文で使用されています。
- Saga Y et al. The cytosolic lncRNA dutA affects STATa signaling and developmental commitment in Dictyostelium. Genes Cells 28:111-128 (2023). PubMed: 36504347
- Umebayashi M et al. A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins. J Cell Biol 222:N/A (2023). PubMed: 36571579
- Beznoussenko GV et al. The Diffusion Model of Intra-Golgi Transport Has Limited Power. Int J Mol Sci 24:N/A (2023). PubMed: 36674888
- Collins C et al. Tubulin acetylation promotes penetrative capacity of cells undergoing radial intercalation. Cell Rep 36:109556 (2021). PubMed: 34407402
- Piao CS et al. Depression following traumatic brain injury in mice is associated with down-regulation of hippocampal astrocyte glutamate transporters by thrombin. J Cereb Blood Flow Metab 39:58-73 (2019). PubMed: 29135354