Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790)
Key features and details
- Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Fr
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Frmore details -
免疫原
mouse IgG whole molecule
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標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Anti-Mouse Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6790の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/200 - 1/1000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| WB |
1/1000 - 1/4000.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/15000.
|
|
| IHC-P |
1/200 - 1/1000.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
1/200 - 1/1000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/4000. |
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/15000. |
|
IHC-P
1/200 - 1/1000. |
|
IHC-Fr
Use at an assay dependent concentration. |
画像
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Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790)Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790) at 1/1000 dilution + Mouse IgG
Observed band size: 28,55 kDa why is the actual band size different from the predicted?Incubated with ab6790 for 60 min at room temperature.
Blocked for 30 min at room temperature.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab6790 は 8 報の論文で使用されています。
- Palomino J et al. Embryo Buoyancy and Cell Death Gene Expression During Embryogenesis of Yellow-Tail Kingfish Seriola lalandi. Front Cell Dev Biol 9:630947 (2021). PubMed: 33816479
- Trainor BM et al. Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae. G3 (Bethesda) 11:N/A (2021). PubMed: 34849786
- Khalid H et al. Discovery of novel Hepatitis C virus inhibitor targeting multiple allosteric sites of NS5B polymerase. Infect Genet Evol 84:104371 (2020). PubMed: 32485331
- Popova S et al. Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal. Sci Rep 10:15185 (2020). PubMed: 32938992
- Zhao X et al. MicroRNA-939-5p directly targets IGF-1R to inhibit the aggressive phenotypes of osteosarcoma through deactivating the PI3K/Akt pathway. Int J Mol Med 44:1833-1843 (2019). PubMed: 31545400
- Li X et al. Effects of in Utero Exposure to Dicyclohexyl Phthalate on Rat Fetal Leydig Cells. Int J Environ Res Public Health 13:N/A (2016). IHC . PubMed: 26907321
- Anttila K et al. Variation in temperature tolerance among families of Atlantic salmon (Salmo salar) is associated with hypoxia tolerance, ventricle size and myoglobin level. J Exp Biol 216:1183-90 (2013). PubMed: 23487268
- Huang HY et al. The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Dev Dyn 238:232-40 (2009). PubMed: 19097054