Goat Anti-Mouse IgG H&L (ab6708)
Key features and details
- Goat Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgG H&L
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details -
免疫原
Mouse IgG, whole molecule
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6708の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/25000.
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IP |
Use at an assay dependent concentration.
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Immunodiffusion |
Use at an assay dependent concentration.
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|
Conjugation |
Use at an assay dependent concentration.
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|
ICC/IF |
Use at an assay dependent concentration.
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|
IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
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|
WB |
1/3000 - 1/15000.
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特記事項 |
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ELISA
1/25000. |
IP
Use at an assay dependent concentration. |
Immunodiffusion
Use at an assay dependent concentration. |
Conjugation
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/3000 - 1/15000. |
画像
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Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg
Secondary
Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
Observed band size: 28,55 kDa why is the actual band size different from the predicted?ab6708 incubation overnight at 4°C.
Both heavy (55kDa) and light (28kDa) chains are detected.
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All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
Lane 1 : Mouse IgG (whole molecule)
Lane 2 : Mouse IgG Fc fragment
Lane 3 : Mouse IgG Fab fragment
Lane 4 : Mouse IgM kappa
Lane 5 : Mouse serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilutionab6708 incubated for 1 hour at room temperature.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (63)
ab6708 は 63 報の論文で使用されています。
- Lee S et al. Oomycete effector AVRblb2 targets cyclic nucleotide-gated channels through calcium sensors to suppress pattern-triggered immunity. New Phytol 241:1277-1291 (2024). PubMed: 38013595
- Verschoor YL et al. Neoadjuvant atezolizumab plus chemotherapy in gastric and gastroesophageal junction adenocarcinoma: the phase 2 PANDA trial. Nat Med 30:519-530 (2024). PubMed: 38191613
- Yick LW et al. T follicular helper cells contribute to pathophysiology in a model of neuromyelitis optica spectrum disorders. JCI Insight 8:N/A (2023). PubMed: 36649074
- Li Y et al. Tumor Cell Nanovaccines Based on Genetically Engineered Antibody-Anchored Membrane. Adv Mater 35:e2208923 (2023). PubMed: 36715052
- Li T et al. Nanoparticle-mediated TRPV1 channel blockade amplifies cancer thermo-immunotherapy via heat shock factor 1 modulation. Nat Commun 14:2498 (2023). PubMed: 37120615