AB98712

Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed

Name: Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (ab98712) | Abcam
Brand: Abcam Limited
SKU: AB98712
Price: 41000 JPY
Availability: InStock

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(2 Publications)

Suitable for IHC-P, ICC/IF, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

別名を表示する

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

4 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling RILPL1 with ab307294 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing centrosome and cytoplasmic staining in SH-SY5Y cell line. Low expression : U-937. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml) followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/400 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307294 at a 1/500 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab98712 at a 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAB8A KO HeLa (RAB8A knockout human cervical adenocarcinoma epithelial cell) (ab264993) cells labeling RAB8A with ab307607 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in Parental HeLa cell line, and no staining in RAB8A KO HeLa cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/50 dilution (10 µg/ml) (Red). Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling RAB8A with ab307607 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in A549 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/50 dilution (10 ug/ml) (Red). Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling RILPL1 with ab307294 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing centrosome staining in Neuro-2a cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml) followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/400 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307294 at a 1/500 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab98712 at a 1/1000 dilution.

Key facts

宿主種

Goat

ターゲット種

Mouse

ターゲットアイソタイプ

IgG

ターゲット特異性

Fc region

最小限の交差反応性

Rat, Human

血清吸着処理済み

Yes

標識

DyLight® 488

励起波長/蛍光波長

Ex: 493nm, Em: 518nm

アプリケーション

ICC/IF, IHC-P, Flow Cyt

applications

クローン性

Polyclonal

アイソタイプ

IgG

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left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Ighg1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Pest management science 80:5364-5376 PubMed39031631

2024

Transcriptome-wide N-methyladenosine profiling reveals growth-defense trade-offs in the response of rice to brown planthopper (Nilaparvata lugens) infestation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Li,Xinyang Tan,Zhen He,Lei Jiang,Yali Li,Liu Yang,Ary A Hoffmann,Chunqing Zhao,Jichao Fang,Rui Ji

Genome biology 22:189 PubMed34167554

2021

The dynamics of N-methyladenine RNA modification in interactions between rice and plant viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhang,Xinjian Zhuang,Zhuozhuo Dong,Kai Xu,Xijun Chen,Fang Liu,Zhen He

View all publications

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Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed