Goat Anti-Human IgG H&L (Biotin) preadsorbed (ab7152)
Key features and details
- Goat Anti-Human IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Goat
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB
製品の概要
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製品名
Goat Anti-Human IgG H&L (Biotin) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Human -
特異性
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
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アプリケーション
適用あり: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WBmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details -
免疫原
Full length native human IgG (purified).
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Secondary antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
ポリ/モノ
ポリクローナル -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | ||
Dot blot | ||
ELISA | ||
IHC-P | ||
Immunomicroscopy | ||
WB |
特記事項 |
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ELISA: 1/20000 - 1/80000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab7152 は 4 報の論文で使用されています。
- Zhang YN et al. Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidates. Nat Commun 14:1985 (2023). PubMed: 37031217
- Zhang YN et al. Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants. bioRxiv N/A:N/A (2021). PubMed: 33791704
- Zhang YN et al. Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants. Sci Adv 7:eabj3107 (2021). PubMed: 34669468
- Limberis MP et al. Intranasal antibody gene transfer in mice and ferrets elicits broad protection against pandemic influenza. Sci Transl Med 5:187ra72 (2013). Human . PubMed: 23720583