Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Goat Anti-Human IgE epsilon chain (ab9159)

Submit a review Q&A (4)References (5)

Key features and details

  • Goat Anti-Human IgE epsilon chain
  • Host species: Goat
  • Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WB

製品の概要

  • 製品名

    Goat Anti-Human IgE epsilon chain
    IgE 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Human

  • 特異性

    The affinity purified anti IgE reacts only with IgE ( several different IgE kappa and lambda myeloma proteins) by IEP and gel diffusion techniques.

  • アプリケーション

    適用あり: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details

  • 免疫原

    Goats were immunized with a mixture of several highly purified human IgE myeloma proteins in Freund’s adjuvant

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.09% Sodium azide
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Serum was collected and passed through several non-IgE containing immunoaffinity supports to make the preparation delta chain specific. The anti delta was immunoaffinity purified off an immunoabsorbant containing a different IgE than was used for immunization thus minimizing idiotypic influences.

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9159の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Immunoelectrophoresis
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Immunoelectrophoresis
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab9159 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9159 は 5 報の論文で使用されています。

  • Malpica L  et al. Regulatory T cells and IgE expression in duodenal mucosa of Strongyloides stercoralis and human T lymphotropic virus type 1 co-infected patients. PLoS Negl Trop Dis 13:e0007415 (2019). PubMed: 31170141
  • Zhang K  et al. Inhibition of Allergic Reactivity through Targeting FceRI-Bound IgE with Humanized Low-Affinity Antibodies. J Immunol 203:2777-2790 (2019). PubMed: 31636239
  • Vanshylla K  et al. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep 8:4244 (2018). PubMed: 29523808
  • Zhang K  et al. Blocking Allergic Reaction through Targeting Surface-Bound IgE with Low-Affinity Anti-IgE Antibodies. J Immunol 198:3823-3834 (2017). PubMed: 28396318
  • Olivier CE  et al. In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics (Sao Paulo) 67:1171-9 (2012). PubMed: 23070344

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Question

What is the isotype of ab9159? Is it possible to obtain ~2 mg in PBS only (no sodium azide? Do you have any anti-human IgE abs, fab fragment, not rabbit host, immunogen affinity purified?

Read More
Answer

Thank you for your patience. ab9159 is a Goat IgG antibody. The specific isotype has not been determined. We can offer the antibody without azide, but the minimum order is 5 mg. Unfortunately we do not have any other anti-human IgE abs that are an fab fragment, non-rabbit host and immunogen affinity purified. Please let me know if there is any other information needed.  

Read More

Question

Dear Sirs, I would like to use the below article for an ELISA to detect IgE. Can you let me know if it is cross-reactive with cynomolgus monkey IgE? Goat polyclonal Secondary Antibody to Human IgE - epsilon chain (ab9159) Best regards      

Read More
Answer

Thank you for contacting us and for your interest in our products. I am sorry to confirm that as far as we are aware, ab9159 Goat polyclonal Secondary Antibody to Human IgE ] has never been tested with samples from cynomolgus monkey. All tested and species cross-reactivity information covered by our guarantee is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.  

Read More

Question

Can you tell me how this antibody performs in a Western Blot and the dilution range recommended by your company. Thanks

Read More
Answer

Thank you for your enquiry. This secondary antibody has been tested for application in Western blotting and I would recommend starting with a dilution of 1:1000 and optimize based upon results. Please note that our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If you have any additional questions, please contact us again.

Read More

Question

I just have a short question. Do you have a goat polyclonal to Human IgE conjugated to AP or HRP in your catalog? We are working with allergenes and will set up Western Blotting. Is this anything you can help us with?

Read More
Answer

Thank you for your enquiry. We currently do not have a Human IgE polyclonal antibody that is conjugated to HRP or AP. We do have a monoclonal: ab7426 - Mouse monoclonal (GE-1) to Human IgE (alkaline phosphatase). If you have any more questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com