Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed

• Flow Cyt

Supplier Data

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling CEACAM1 + CEACAM3 + CEACAM6 with ab104450 at 1/1000 dilution (0.1&micor;g) followed by a secondary antibody Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161)used at a 1/2000 dilution (Red) compared with an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : HeLa (PMID : 11580753)
Gated on viable cells.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) cells labeling Myeloperoxidase with ab300650 at 1/50 dilution (20.26 µg/ml), followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in HL-60 cell line. Negative control : HeLa (PMID : 12040446). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab300650 at a 1/50 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab150161 at a 1/1000 dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / HL-60 (human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with ab300650 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : HeLa (PMID : 12040446).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y(human neuroblastoma epithelial cell, Left) / MCF7(human breast adenocarcinoma epithelial cell, Right) cells labelling Cytokeration 19 with ab323561 at 1/1000 dilution (0.1 ug) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/5000 dilution was used as the secondary antibody.

Negative control : SH-SY5Y.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (green) was ab150161 Alexa Fluor® 488 goat anti-rat IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Composite multiplex immunofluorescence staining of Iba1, GFAP and MAP2 staining in a section of formalin-fixed paraffin-embedded human cerebral cortex*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (Ph6.0) using retrieval settings of 100°C for 20 minutes. The section was then incubated at room temperature for 1 hour with ab300156 at 1µg/ml dilution (shown in green), ab183830 at 1µg/ml (shown in magenta), and ab302644 at 1µg/ml (shown in yellow). Then incubated for 1 hour with ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed 1/1000, ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed 1/1000, and ab150134 Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed 1/1000. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with the EVOS™ S1000 Spatial Imaging System (ThermoFisher Scientific) with spectral unmixing and minor subsequent contrast adjustment.

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling ATG16L1 with ab309495 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing increased cytoplasmic staining in MEF cells after treatment with amino acid and serum starvation in HBSS for 3 h. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using ab8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : WEHI-231.(PMID : 2457584) Gated on viable cells.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized mouse blood cells labeling Myeloperoxidase with ab300650 at 1/1000 dilution (0.1µg)/ Right compared with a rat monoclonal IgG / Left. Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Composite multiplex immunofluorescence staining of ab279297, ab317042 and ab308439 staining NeuN, NEFH and Tau (MBD region) in Mouse Primary Neurons DIV14 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab279297 (shown in green), ab317042 (shown in magenta) and ab308439 (shown in yellow) at 1µg/ml. Cells were then incubated with ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed, ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed and ab150084 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow Cytometry analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CD68 with ab53444 at a 1/100 dilution (1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt

Supplier Data

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of L-929 (mouse connective tissue fibroblast, Left) / EL4 (mouse lymphoma T lymphocyte, Right) cells labelling PD1 with ab309360 at 1/1000 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/5000 dilution was used as the secondary antibody. Negative control : L-929 (PMID : 1396582). Gated on viable cells.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing cytoplasmic staining in mouse splenocytes (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast, Left) / WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD45 with ab25386 at 1/500 dilution (0.1 µg) / Right compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and Cell without incubation with primary antibody and secondary antibody / Blue.

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) was used as a secondary antibody at a 1/5000 dilution.

Negative control : Neuro-2a.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Mouse PBMC cells labelling CD45 with ab25386 at 1/500 dilution (0.1 µg) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) was used as a secondary antibody at a 1/5000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing membranous staining in WEHI-231 cell line (shown in green), no staining was observed in Neuro-2a cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control : Neuro-2a.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing membranous staining in J774A.1 cell line (shown in green), no staining was observed in C2C12 cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control : C2C12.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

• Flow Cyt

Supplier Data

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of A20 (mouse reticulum sarcoma B lymphocyte) cells labelling Transferrin Receptor with ab256568 at 1/500 dilution (0.1μg) (Red) compared with a Rat monoclonal IgG2a (ab18450) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt

Supplier Data

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of mouse thymocytes labeling C-C chemokine receptor type 9 with ab303674 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow Cytometry analysis of mouse thymocytes (right panel) compared to a rat monoclonal IgG isotype control (left panel). ab303674 used at a 1/1000 dilution. Secondary is Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) used at a 1/2000 dilution. Cells were co-stained with APC conjugated anti-CD4. The conjugation was performed by using ab251480 and APC Conjugation Kit - Lightning-Link® (ab201807). Gated on viable cells.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt

Lab

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling C5a-R with ab117579 at 1/50 dilution (0.1ug) / Right compared with a Rat monoclonal IgG / Left isotype control.

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) was used as a secondary at a 1/5000 dilution.

Gated on viable cells.
Cells were co-stained with anti mouse CD11b conjugated to Brilliant Violet 421.

• Flow Cyt

Lab

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling C5a-R with ab117579 at 1/50 dilution (0.1ug) / Right compared with a Rat monoclonal IgG / Left isotype control.

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) was used as a secondary at a 1/5000 dilution.

Gated on viable cells.
Cells were co-stained with anti mouse CD19 conjugated to Alexa Fluor®647.

• Flow Cyt

Lab

Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)

Flow cytometric analysis of Mouse PBMC (Mouse peripheral blood mononuclear cell) cells labelling CD62L with ab119834 at 1/1000 dilution (0.1 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/5000 dilution was used as the secondary antibody and Rat IgG2a, kappa monoclonal [RTK2758] (ab18450) was used as an Isotype control (Left). Gated on viable cells.

• Alexa Fluor®

Unknown

Alexa Fluor® - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (AB150161)