AB6267

Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed

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(14 Publications)

Suitable for Flow Cyt, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 14 publications.

別名を表示する

Ig gamma-1 chain C region

Key facts

宿主種

Goat

ターゲット種

Rat

ターゲットアイソタイプ

IgG

ターゲット特異性

F(ab')2

最小限の交差反応性

Horse, Cow, Human

血清吸着処理済み

Yes

標識

Texas Red®

励起波長/蛍光波長

Ex: 589nm, Em: 615nm

アプリケーション

IM, Flow Cyt, ICC/IF, ELISA

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製に関する特記事項

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

バッファー組成

pH: 6.5 Preservative: 0.05% Sodium azide Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Bone 151:116058 PubMed34144232

2021

Expression profiling of mitochondria-associated microRNAs during osteogenic differentiation of human MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Zheng,Jin Liu,Jinsheng Yu,Audrey McAlinden

Respiratory research 22:146 PubMed33980216

2021

Relationship between long non-coding RNA PCAT-1 expression and gefitinib resistance in non-small-cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojia Wang,Chao Liu,Qing Lei,Zhengwei Wu,Xiangshuai Miao,Debing Zhu,Xu Yang,Na Li,Mingwei Tang,Yan Chen,Weiwei Wang

Genes & diseases 9:1332-1344 PubMed35873020

2021

SQSTM1/p62 regulate breast cancer progression and metastasis by inducing cell cycle arrest and regulating immune cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Long Qi,Jin-Rong He,Cun-Bao Liu,Shu-Mei Jin,Xu Yang,Hong-Mei Bai,Yan-Bing Ma

Bone 142:115705 PubMed33141070

2020

Delineation of the 1q24.3 microdeletion syndrome provides further evidence for the potential role of non-coding RNAs in regulating the skeletal phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

James L Shepherdson,Hongjun Zheng,Ina E Amarillo,Audrey McAlinden,Marwan Shinawi

Aging 12:17990-18007 PubMed33021962

2020

SIRT1 activation by minocycline on regulation of microglial polarization homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Hsuan Wu,Bor-Ren Huang,Sheng-Wei Lai,Chingju Lin,Hsiao-Yun Lin,Liang-Yo Yang,Dah-Yuu Lu

American journal of translational research 12:940-949 PubMed32269725

2020

Metformin ameliorates bleomycin-induced pulmonary fibrosis in mice by suppressing IGF-1.

Applications

Unspecified application

Species

Unspecified reactive species

Huijuan Xiao,Xiaoxi Huang,Shiyao Wang,Zheng Liu,Run Dong,Dingyun Song,Huaping Dai

Bone 123:92-102 PubMed30898695

2019

MicroRNA-181a/b-1 over-expression enhances osteogenesis by modulating PTEN/PI3K/AKT signaling and mitochondrial metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Zheng,Jin Liu,Eric Tycksen,Ryan Nunley,Audrey McAlinden

JBMR plus 3:e10071 PubMed30828688

2019

MicroRNA-138 Inhibits Osteogenic Differentiation and Mineralization of Human Dedifferentiated Chondrocytes by Regulating RhoC and the Actin Cytoskeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Zheng,David Ramnaraign,Britta A Anderson,Eric Tycksen,Ryan Nunley,Audrey McAlinden

American journal of translational research 10:2967-2974 PubMed30323883

2018

Ouabain ameliorates bleomycin induced pulmonary fibrosis by inhibiting proliferation and promoting apoptosis of lung fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Biyun Li,Xiaoxi Huang,Zheng Liu,Xuefeng Xu,Huijuan Xiao,Xin Zhang,Huaping Dai,Chen Wang

Journal of molecular medicine (Berlin, Germany) 96:391-402 PubMed29502203

2018

Amantadine attenuates sepsis-induced cognitive dysfunction possibly not through inhibiting toll-like receptor 2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xing,Pinjie Huang,Yang Lu,Weian Zeng,Zhiyi Zuo

View all publications

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Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed