Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed (ab6517)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Related conjugates and formulations
製品の概要
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製品名
Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
特異性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
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アプリケーション
適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
吸着処理血清
Cow, Horse, Human more details -
免疫原
Rat IgG F(ab’)2 fragment
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標識
HRP
製品の特性
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製品の状態
Lyophilized:Reconstitute with 0.5 ml of water. -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituents: 1.5% BSA, 0.164% Sodium phosphate, 1.46% Sodium chloride
BSA is IgG and protease free -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項（標識）
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/5000.
|
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| Dot blot |
Use at an assay dependent dilution.
|
|
| WB |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/100000.
|
|
| Electron Microscopy |
Use at an assay dependent dilution.
|
|
| IHC |
Use at an assay dependent dilution.
|
| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/5000. |
|
Dot blot
Use at an assay dependent dilution. |
|
WB
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/100000. |
|
Electron Microscopy
Use at an assay dependent dilution. |
|
IHC
Use at an assay dependent dilution. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab6517 は 3 報の論文で使用されています。
- Motomura E et al. Cell-autonomous signal transduction in the Xenopus egg Wnt/ß-catenin pathway. Dev Growth Differ 56:640-52 (2014). WB . PubMed: 25330272
- Wang SH et al. Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins. Glycobiology N/A:N/A (2013). PubMed: 23363740
- Engelson EJ et al. An Essential Farnesylated Kinesin in Trypanosoma brucei. PLoS One 6:e26508 (2011). WB . PubMed: 22073170
