Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed (ab6012)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopy
Related conjugates and formulations
製品の概要
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製品名
Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
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アプリケーション
適用あり: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Rabbit IgG whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項（標識）
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6012の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
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| IHC-P |
Use at an assay dependent dilution. PubMed: 21498392
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| Dot blot |
Use at an assay dependent dilution.
|
|
| WB |
1/2000 - 1/10000.
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| ELISA |
1/20000 - 1/100000.
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|
| IHC |
Use at an assay dependent dilution.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
| 特記事項 |
|---|
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ICC/IF
1/1000 - 1/5000. |
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IHC-P
Use at an assay dependent dilution. PubMed: 21498392 |
|
Dot blot
Use at an assay dependent dilution. |
|
WB
1/2000 - 1/10000. |
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ELISA
1/20000 - 1/100000. |
|
IHC
Use at an assay dependent dilution. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (10)
ab6012 は 10 報の論文で使用されています。
- Siebert-Kuss LM et al. Transcriptome analyses in infertile men reveal germ cell-specific expression and splicing patterns. Life Sci Alliance 6:N/A (2023). PubMed: 36446526
- Di Persio S et al. Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Rep Med 2:100395 (2021). PubMed: 34622232
- Silva AF et al. Effects of chronic moderate alcohol consumption on right ventricle and pulmonary remodelling. Exp Physiol 106:1359-1372 (2021). PubMed: 33605491
- Pohl E et al. Ageing in men with normal spermatogenesis alters spermatogonial dynamics and nuclear morphology in Sertoli cells. Andrology 7:827-839 (2019). PubMed: 31250567
- Webb TE et al. An internal ribosome entry site in the 5' untranslated region of epidermal growth factor receptor allows hypoxic expression. Oncogenesis 4:e134 (2015). WB ; Human . PubMed: 25622307
- Huang R et al. Association between human cytomegalovirus infection and histone acetylation level in various histological types of glioma. Oncol Lett 10:2812-2820 (2015). IHC . PubMed: 26722247
- van der Poorten D et al. Hepatic fat loss in advanced nonalcoholic steatohepatitis: are alterations in serum adiponectin the cause? Hepatology 57:2180-8 (2013). PubMed: 22996622
- Wang Z et al. Changes in large pulmonary arterial viscoelasticity in chronic pulmonary hypertension. PLoS One 8:e78569 (2013). IHC (PFA fixed) . PubMed: 24223157
- Kacevska M et al. Extrahepatic cancer suppresses nuclear receptor-regulated drug metabolism. Clin Cancer Res 17:3170-80 (2011). IHC-P . PubMed: 21498392
- Chamberlain G et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt . PubMed: 18698345
