AB6012

Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed

Name: Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed (ab6012) | Abcam
Brand: Abcam Limited
SKU: AB6012
Price: 42000 JPY
Availability: InStock

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(15 Publications)

Goat F(ab')2 Anti-Rabbit IgG - H&L(Biotin)(ab6012) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC and ELISA.

- Preadsorbed to minimize non-specific binding and high background staining
- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Key facts

宿主種

Goat

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Hamster, Cow, Human, Horse

血清吸着処理済み

Yes

標識

Biotin

励起波長/蛍光波長

アプリケーション

ICC/IF, IHC, IM, Dot, ELISA, WB, IHC-P

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/100000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 17:1417-1451 PubMed40374915

2025

Genotype-specific differences in infertile men due to loss-of-function variants in M1AP or ZZS genes.

Applications

Unspecified application

Species

Unspecified reactive species

Nadja Rotte,Jessica E M Dunleavy,Michelle D Runkel,Lina Bosse,Daniela Fietz,Adrian Pilatz,Johanna Kuss,Ann-Kristin Dicke,Sofia B Winge,Sara Di Persio,Christian Ruckert,Verena Nordhoff,Hans-Christian Schuppe,Kristian Almstrup,Sabine Kliesch,Nina Neuhaus,Birgit Stallmeyer,Moira K O'Bryan,Frank Tüttelmann,Corinna Friedrich

Frontiers in endocrinology 15:1483364 PubMed39758349

2025

Umbilical vein remodeling is associated with pregestational maternal overweight.

Applications

Unspecified application

Species

Unspecified reactive species

Kamilla Batista da Silva Souza,Luana Caroline Hochberger,Felippe Egon Castrignano Camargo,Gabriely Santos Silva,Giovanna Castrignano Camargo,João Pedro Lourenço Mello,Fernanda Cristina Alcantara Dos Santos,Fernanda Regina Giachini,Núbia de Souza Lobato,Paula Cristina de Souza Souto

Human reproduction (Oxford, England) 39:892-901 PubMed38365879

2024

Identification of two hidden clinical subgroups among men with idiopathic cryptozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Charlotte Schülke,Joachim Wistuba,Verena Nordhoff,Hermann M Behre,Jann-Frederik Cremers,Sabine Kliesch,Sara Di Persio,Nina Neuhaus

Molecular cell 84:1021-1035.e11 PubMed38359823

2024

C19ORF84 connects piRNA and DNA methylation machineries to defend the mammalian germ line.

Applications

Unspecified application

Species

Unspecified reactive species

Ansgar Zoch,Gabriela Konieczny,Tania Auchynnikava,Birgit Stallmeyer,Nadja Rotte,Madeleine Heep,Rebecca V Berrens,Martina Schito,Yuka Kabayama,Theresa Schöpp,Sabine Kliesch,Brendan Houston,Liina Nagirnaja,Moira K O'Bryan,Kenneth I Aston,Donald F Conrad,Juri Rappsilber,Robin C Allshire,Atlanta G Cook,Frank Tüttelmann,Dónal O'Carroll

Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 17:32-42 PubMed36746123

2023

Testicular Architecture of Men with 46,XX Testicular Disorders of Sex Development.

Applications

Unspecified application

Species

Unspecified reactive species

Mirkka Hiort,Julia Rohayem,Regine Knaf,Sandra Laurentino,Agnethe Berglund,Claus H Gravholt,Jörg Gromoll,Joachim Wistuba

Life science alliance 6: PubMed36446526

2022

Transcriptome analyses in infertile men reveal germ cell-specific expression and splicing patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Lara M Siebert-Kuss,Henrike Krenz,Tobias Tekath,Marius Wöste,Sara Di Persio,Nicole Terwort,Margot J Wyrwoll,Jann-Frederik Cremers,Joachim Wistuba,Martin Dugas,Sabine Kliesch,Stefan Schlatt,Frank Tüttelmann,Jörg Gromoll,Nina Neuhaus,Sandra Laurentino

Cell reports. Medicine 2:100395 PubMed34622232

2021

Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Di Persio,Tobias Tekath,Lara Marie Siebert-Kuss,Jann-Frederik Cremers,Joachim Wistuba,Xiaolin Li,Gerd Meyer Zu Hörste,Hannes C A Drexler,Margot Julia Wyrwoll,Frank Tüttelmann,Martin Dugas,Sabine Kliesch,Stefan Schlatt,Sandra Laurentino,Nina Neuhaus

Experimental physiology 106:1359-1372 PubMed33605491

2021

Effects of chronic moderate alcohol consumption on right ventricle and pulmonary remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Filipa Silva,Fábio Sousa-Nunes,Gabriel Faria-Costa,Ilda Rodrigues,João Tiago Guimarães,Adelino Leite-Moreira,Tiago Henriques-Coelho,Rita Negrão,Daniel Moreira-Gonçalves

Andrology 7:827-839 PubMed31250567

2019

Ageing in men with normal spermatogenesis alters spermatogonial dynamics and nuclear morphology in Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

E Pohl,V Höffken,S Schlatt,S Kliesch,J Gromoll,J Wistuba

Oncology letters 10:2812-2820 PubMed26722247

2015

Association between human cytomegalovirus infection and histone acetylation level in various histological types of glioma.

Applications

IHC

Species

Unspecified reactive species

Rui Huang,Dongmeng Qian,Ming Hu,Xue Zhang,Jingyi Song,Ling Li,Hao Chen,Bin Wang

View all publications

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Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed