Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase) (ab6024)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
Related conjugates and formulations
製品の概要
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製品名
Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab) .
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アプリケーション
適用あり: ICC/IF, Dot blot, WB, ELISA, IHCmore details -
免疫原
Rabbit IgG F(ab')2 fragment
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標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
F(ab')2 Anti-Rabbit IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6024の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | ||
Dot blot | ||
WB | ||
ELISA | ||
IHC |
特記事項 |
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ELISA: 1/600 - 1/2800.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2800 of the reconstitution concentration is suggested for this product.
Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab6024 は論文での使用が確認できていません。