AB6112

Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed

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(47 Publications)

Suitable for WB, Dot, EM, IHC, ICC, ELISA, IHC-P. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 47 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Unknown

Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

ab6112 was used at dilution 1/5000 with the primary antibody ab32117 in WB. See the review on ab32117.

Secondary

All lanes:

Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)

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Key facts

宿主種

Goat

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

F(ab')2

最小限の交差反応性

Mouse, Rat, Sheep, Horse, Cow, Human

血清吸着処理済み

Yes

標識

HRP

励起波長/蛍光波長

アプリケーション

EM, IHC, WB, ICC, Dot, ELISA, IHC-P

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100000", "notes":"This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity." }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500", "notes":" Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Anti-Rabbit IgG F(ab')2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

バッファー組成

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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文献 (47)

Recent publications for all applications. Explore the full list and refine your search

Toxicological sciences : an official journal of the Society of Toxicology 198:288-302 PubMed38290791

2024

Exploring the effects of topoisomerase II inhibitor XK469 on anthracycline cardiotoxicity and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Keresteš,Jan Kubeš,Lenka Applová,Petra Kollárová,Olga Lenčová-Popelová,Iuliia Melnikova,Galina Karabanovich,Mushtaq M Khazeem,Hana Bavlovič-Piskáčková,Petra Štěrbová-Kovaříková,Caroline A Austin,Jaroslav Roh,Martin Štěrba,Tomáš Šimůnek,Anna Jirkovská

Acta biochimica et biophysica Sinica 55:866-877 PubMed37184280

2023

Monomethyl fumarate prevents alloimmune rejection in mouse heart transplantation by inducing tolerogenic dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juntao Chen,Yirui Cao,Ouyang Jia,Xuanchuan Wang,Yongsheng Luo,Yin Celeste Cheuk,Tongyu Zhu,Dong Zhu,Yi Zhang,Jina Wang

Folia neuropathologica 61:53-67 PubMed37114961

2023

HSF1 inhibits microglia activation to attenuate neuroinflammation via regulating miR-214-3p and NFATc2 in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuangao Liao,Yong Gu,Jinhua Wang,Yu Tian,Xiaohong Ni,Lei Zhou,Ye Ye,Guangming Xia

World journal of gastrointestinal oncology 14:2353-2366 PubMed36568946

2022

Anti-silencing function 1B knockdown suppresses the malignant phenotype of colorectal cancer by inactivating the phosphatidylinositol 3-kinase/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gen-Hua Yu,Xu-Feng Gong,Ying-Ying Peng,Jun Qian

American journal of translational research 14:8129-8145 PubMed36505286

2022

MiR-216b targets CPEB4 to suppress colorectal cancer progression through inhibiting IL-10-mediated M2 polarization of tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zou,Boyu Wu,Chao Lin,Qunhua Ding,Jiangtao Li

International journal of molecular sciences 23: PubMed36430510

2022

Deciphering the Role and Signaling Pathways of PKCα in Luminal A Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emilio M Serrano-López,Teresa Coronado-Parra,Consuelo Marín-Vicente,Zoltan Szallasi,Victoria Gómez-Abellán,María José López-Andreo,Marcos Gragera,Juan C Gómez-Fernández,Rubén López-Nicolás,Senena Corbalán-García

Cell biology international 46:1959-1969 PubMed35930601

2022

Silencing of COL3A1 represses proliferation, migration, invasion, and immune escape of triple negative breast cancer cells via down-regulating PD-L1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Ling Lin,Xiaohua Li,Ronglan Wen,Xin Zhang

Experimental & molecular medicine 54:1024-1037 PubMed35879346

2022

Circular RNA ROCK1, a novel circRNA, suppresses osteosarcoma proliferation and migration via altering the miR-532-5p/PTEN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Liu,Guanzhen Qiu,Yinzhou Luo,Shanshan Li,Yeqiu Xu,Yuanzhuang Zhang,Jiayuan Hu,Peifeng Li,Hai Pan,Yong Wang

Molecular medicine (Cambridge, Mass.) 28:29 PubMed35255803

2022

Gene therapy of yeast NDI1 on mitochondrial complex I dysfunction in rotenone-induced Parkinson's disease models in vitro and vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Hongzhi Li,Bohao Sun,Yuting Huang,Jing Zhang,Xuejing Xu,Yuqi Shen,Zhuo Chen,Jifeng Yang,Luxi Shen,Yongwu Hu,Haihua Gu

Molecular medicine reports 25: PubMed35039876

2022

Comparison of the effect of rotenone and 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine on inducing chronic Parkinson's disease in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Bohao Sun,Jifeng Yang,Zhuo Chen,Zhengzheng Li,Nan Zhang,Hongzhi Li,Luxi Shen

View all publications

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Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed