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Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

Submit a review Q&A (2)References (3)
Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

    Key features and details

    • Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
    • Conjugation: Biotin
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cyt

    製品の概要

    • 製品名

      Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
      IgM 二次抗体 製品一覧

    • 由来種

      Goat

    • ターゲット生物種

      Mouse

    • 特異性

      No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  No reaction was observed against other mouse heavy or light chain proteins.

    • アプリケーション

      適用あり: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details

    • 免疫原

      Mouse IgM mu heavy chain

    • 標識

      Biotin

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
    • Concentration information loading...

    • 精製度

      Affinity purified

    • 特記事項（精製）

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    • 特記事項（標識）

      Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab5929の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/50000.
    WB
    1/2000 - 1/10000.
    Dot blot
    Use at an assay dependent concentration.
    IHC
    1/1000 - 1/5000.
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    1/200 - 1/1000.
    特記事項
    ICC/IF
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/50000.
    WB
    1/2000 - 1/10000.
    Dot blot
    Use at an assay dependent concentration.
    IHC
    1/1000 - 1/5000.
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    1/200 - 1/1000.

    画像

    • Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
      Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

      ab5929 was used as the secondary antibody in WB.

      See Abreview

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (3)

    ab5929 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5929 は 3 報の論文で使用されています。

    • Liu HY  et al. Leveraging the Treg-intrinsic CTLA4-PKC? signaling pathway for cancer immunotherapy. J Immunother Cancer 9:N/A (2021). PubMed: 34588224
    • Hong R  et al. Distinct roles of BCNP1 in B-cell development and activation. Int Immunol 32:17-26 (2020). PubMed: 31412363
    • Grewal N  et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . PubMed: 25502628

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question


    I have some questions for you. Recently, I tried to use your antibodies, ab49394(primary AB) and ab5929( 2nd antibody), doing IHC for measure macrophages of small intestine tissue of human for a few times. I got good stains for positive samples without background, but negative control(without primary antibody)also got some positive spots. I tried to block it, I used:
    1. 5% BSA in TBS with tween 20, 2 hours at RT;
    2. 5% normal goat serum in TBS, 2 hours at RT;
    3. I also blocked endogenous Avidin used vector Lab,SP-2002 and endogenous HRP used Dako, S2003.
    Could you give me some suggestions to solute this problem?

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    Answer

    Thank you for contacting Abcam.

    I am sorry that you are experiencing some issues with one of our antibodies. To be able to help further in this matter, would you be able to supply some images of the staining that you are with both your positive and negative sections. By being able to see the staining that you are getting, I will hopefully be able to resolve this issue quickly for you.

    I look forward to your reply.

    Read More

    Question

    I am working with B cells which express an human IgG or human IgM as well as a murine IgM. I would like to show the expression of the murine IgM in FACS. Therefore I need an antibody which ONLY recognizes the murine IgM but not the human IgM or IgG. In your catalogue I found an "Mouse IgM secondary antibody - µ chain speciic- F(ab)2 Fragment (ab5932). Do you know if this antibody crossreacts with human IgM/G? Unfortunately this antibody is coupled to PE. Since my cells are GFP positive I prefere Biotin or APC labeled antibodies. Do you have an anti mouse-µ antibody not reacting with human IgG/M which is coupled to Biotin or APC?

    Read More
    Answer

    We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cross adsorbed or tested against human IgM, and we cannot guarantee their suitable for your assay. Two other biotin conjugated anti-mouse IgG antibodies but only tested in ELISA: ab11659 Mouse IgM secondary antibody [LO-MM-9] - Heavy Chain specific. Unable to guarantee non-reactivity with human Ig. ab49760 will not recognise other mouse Ig's as there was no reaction against IgA, IgG, and IgM. Human IgG or IgM cross-reactivity has not been tested.

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